Spontaneous recombinase activity of Cre-ERT2 in vivo
Autor: | Ryley K. Zastrow, Abigail B. Radcliff, Robert D. Blank, Jasmin Kristianto, Michael G. Johnson |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Genetically modified mouse Estrogen receptor Cre recombinase Chromosomal translocation Mice Transgenic Biology Endothelin-Converting Enzymes Article 03 medical and health sciences 0302 clinical medicine Genetics Recombinase Animals Estrogen Receptor beta Transgenes Mice Knockout Recombinase activity Integrases Gene targeting Molecular biology Mice Inbred C57BL Tamoxifen 030104 developmental biology Cytoplasm Animal Science and Zoology Agronomy and Crop Science 030217 neurology & neurosurgery Biotechnology |
Zdroj: | Transgenic Res |
ISSN: | 1573-9368 |
Popis: | Inducible Cre-ERT recombinase technology is widely used for gene targeting studies. The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg(UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT. The putative mechanism of selective Cre-mediated recombination is Cre sequestration in the cytoplasm in the basal state with translocation to the nucleus only in the presence of tamoxifen. We utilized both a reporter mouse (B6.129 (Cg)-Gt(ROSA)26Sor(tm4(ACTB-tdTomato,-EGFP)Luo)/J) and endothelin converting enzyme-1 (Ece1) floxed transgenic mouse line to evaluate Cre-ERT2 activity. We observed spontaneous Cre activity in both settings. Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation. Thus, it is important to consider background Cre activity in experimental design. |
Databáze: | OpenAIRE |
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