Molecular detection of serotype groups of Listeria monocytogenes isolated from gallbladder of cattle and sheep in Iraq
| Autor: | Zainab Jaber Hadi, Ali M. Almohana, Hamza Jawad Al-Ali, Alaa H. Al-Charrakh, Samer A. Al-Hilali, Mohsen A. Alrodhan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype sheep Veterinary medicine 030106 microbiology Biology medicine.disease_cause SF1-1100 Microbiology law.invention 03 medical and health sciences Listeria monocytogenes law SF600-1100 medicine Pathogen Gene gallbladder Polymerase chain reaction General Veterinary Gallbladder Hemolysin Animal culture molecular detection 030104 developmental biology medicine.anatomical_structure cattle biology.protein Flagellin |
| Zdroj: | Veterinary World, Vol 11, Iss 4, Pp 431-436 (2018) |
| ISSN: | 2231-0916 0972-8988 |
| DOI: | 10.14202/vetworld.2018.431-436 |
| Popis: | Aim: This study was designed to investigate the occurrence of serotypes of Listeria monocytogenes, an important food-borne pathogen, in gallbladder samples from cattle and sheep. Materials and Methods: Three hundred samples were collected and screened for the presence of L. monocytogenes. The identification of the isolates was confirmed by API-Listeria system and by the presence of hemolysin (hyl) gene. The isolates were subjected to polymerase chain reaction-based serotype identification with d1 (division 1), d2 (division 2), glt, mama (mismatch amplification mutation assay), and flaA (flagellin protein) genes. Results: A total of 8 (2.7%) L. monocytogenes were recovered from 6 (4.0%) samples of sheep and 2 (1.3%) samples of cattle. All isolates showed positive results with Hly primers. Four isolates carried d1 gene, did not possess glt gene and harbored mama gene. The serotypes of these isolates were identified as 4a or 4c. The other 4 isolates carried d2 gene, 3 of them were positive with the FlaA primers, and hence, determined to be a 1/2a or 3a serotype, and 1 isolate was determined to be 1/2c or 3c serotype. Conclusion: This study concluded that the presence of 1/2a serotype in gallbladder samples indicates public health risk through cross-contamination of meat at slaughterhouses. |
| Databáze: | OpenAIRE |
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