Epigallocatechin Gallate-Mediated Alteration of the MicroRNA Expression Profile in 5α-Dihydrotestosterone-Treated Human Dermal Papilla Cells
| Autor: | Hae Jeong Youn, Su Young Kim, In-Sook An, Shanghun Shin, Sung Jin Choi, Karam Kim, Hyunjoo Han, Sungkwan An, Jung-Min Ko, Jeongju Lee, Kyu Joong Ahn, Kyung-Suk Kim, Myung Joo Lee, Hwa Jun Cha |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Programmed cell death Cell cycle checkpoint Dermatology Biology Epigallocatechin gallate urologic and male genital diseases 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Human dermal papilla cells Viability assay integumentary system food and beverages MicroRNA Expression Profile medicine.disease Hair follicle Cell biology MicroRNAs 030104 developmental biology Hair loss Endocrinology medicine.anatomical_structure chemistry Dihydrotestosterone Original Article sense organs hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Annals of Dermatology |
| ISSN: | 2005-3894 1013-9087 |
| DOI: | 10.5021/ad.2016.28.3.327 |
| Popis: | Background Dihydrotestosterone (DHT) induces androgenic alopecia by shortening the hair follicle growth phase, resulting in hair loss. We previously demonstrated how changes in the microRNA (miRNA) expression profile influenced DHT-mediated cell death, cell cycle arrest, cell viability, the generation of reactive oxygen species (ROS), and senescence. Protective effects against DHT have not, however, been elucidated at the genome level. Objective We showed that epigallocatechin gallate (EGCG), a major component of green tea, protects DHT-induced cell death by regulating the cellular miRNA expression profile. Methods We used a miRNA microarray to identify miRNA expression levels in human dermal papilla cells (DPCs). We investigated whether the miRNA expression influenced the protective effects of EGCG against DHT-induced cell death, growth arrest, intracellular ROS levels, and senescence. Results EGCG protected against the effects of DHT by altering the miRNA expression profile in human DPCs. In addition, EGCG attenuated DHT-mediated cell death and growth arrest and decreased intracellular ROS levels and senescence. A bioinformatics analysis elucidated the relationship between the altered miRNA expression and EGCG-mediated protective effects against DHT. Conclusion Overall, our results suggest that EGCG ameliorates the negative effects of DHT by altering the miRNA expression profile in human DPCs. |
| Databáze: | OpenAIRE |
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