Accelerated intervertebral disc degeneration in scoliosis versus physiological ageing develops against a background of enhanced anabolic gene expression
| Autor: | G. Zimmermann, Helge Bertram, Eric Steck, Andreas G. Nerlich, Wiltrud Richter, Bohua Chen, Claus Carstens |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Aging medicine.medical_specialty Adolescent Matrix Metalloproteinases Membrane-Associated Lumican Decorin Biophysics Gene Expression Degeneration (medical) Biochemistry Internal medicine Matrix Metalloproteinase 13 Chondromodulin medicine Humans Collagenases RNA Messenger Child Intervertebral Disc Molecular Biology Aggrecan Aged Oligonucleotide Array Sequence Analysis Tissue Inhibitor of Metalloproteinase-1 business.industry Biglycan Intervertebral disc Cell Biology Anatomy Middle Aged Matrix Metalloproteinases medicine.anatomical_structure Endocrinology Scoliosis Ageing Case-Control Studies Female business Intervertebral Disc Displacement |
| Zdroj: | Biochemical and Biophysical Research Communications. 342:963-972 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.02.048 |
| Popis: | Molecular consequences of long-term deformation and altered mechanical loading of intervertebral disc (IVD) tissue in scoliosis have yet to be elucidated. We hypothesized that histological disc degeneration is faster in scoliosis than in normal ageing and that this is reflected by an altered gene expression profile. A semiquantitative histodegeneration score (HDS) revealed significantly enhanced degeneration in scoliosis (HDS 5.3) versus age-matched control IVDs (HDS 2.25; p = 0.001). Gene expression analysis by cDNA array and RT-PCR demonstrated higher mRNA levels for extracellular-matrix molecules like aggrecan, biglycan, decorin, lumican, chondromodulin, and COL2A1 in scoliotic discs versus normal discs of identical degeneration score. No differences were evident for catabolic molecules like MMP3, MMP13, MMP17, and TIMP1. In sum, morphologic disc degeneration was accelerated by about 2 decades in scoliosis versus physiological ageing and developed against a background of stronger anabolic matrix metabolism at younger age or in response to the altered mechanical environment of the tissue. |
| Databáze: | OpenAIRE |
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