The low doses of SWCNTs affect the expression of proliferation and apoptosis related genes in normal human astrocytes
| Autor: | Dariia O. Tsymbal, Yuliia V. Yefimova, Myroslava Y. Sliusar, Oleksandr H. Minchenko, Dmytro O. Minchenko, Olha V. Rudnytska, Olena O. Khita |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Messenger RNA
Chemistry Health Toxicology and Mutagenesis Endoplasmic reticulum mRNA IGFBP3 MicroRNA Normal human astrocytes Toxicology medicine.disease_cause Applied Microbiology and Biotechnology Article Cell biology SWCNT RA1190-1270 microRNA Toxicology. Poisons medicine Viability assay Binding site Genotoxicity Gene ComputingMethodologies_COMPUTERGRAPHICS |
| Zdroj: | Current Research in Toxicology, Vol 2, Iss, Pp 64-71 (2021) Current Research in Toxicology |
| Popis: | Graphical abstract Highlights • Low doses of SWCNTs affect the expression of numerous genes in normal astrocytes. • Low doses of SWCNTs interrupt the expression of various microRNAs in astrocytes. • SWCNTs affect the gene expressions through induction of endoplasmic reticulum stress. • Low doses of SWCNTs disturb the genome stability showing the genotoxic effects. • SWCNTs at low doses may have potential exposure-related risks for human health. The unique properties of single-walled carbon nanotubes (SWCNTs) make them viable candidates for versatile implementation in the biomedical devices. They are able to cross the blood–brain barrier, enter cells and accumulate in cell nuclei. We studied the effect of these carbon nanoparticles on the expression of genes associated with endoplasmic reticulum stress and proliferation, cell viability and cancerogenesis as well as microRNAs in normal human astrocytes. We have shown that treatment of normal human astrocytes by small doses of SWCNTs (2 and 8 ng/ml of medium for 24 hrs) affect the expression of DNAJB9, IGFBP3, IGFBP6, CLU, ZNF395, KRT18, GJA1, HILPDA, and MEST mRNAs as well as several miRNAs, which have binding sites at 3′-UTR of these mRNAs. These changes in the expression profile of individual mRNAs introduced by SWCNTs are dissimilar in magnitude and direction and are the result of both transcriptional and posttranscriptional mechanisms of regulation. It is possible that these changes in gene expressions are mediated by the endoplasmic reticulum stress introduced by carbon nanotubes and reflect the disturbance of the genome stability. In conclusion, the low doses of SWCNTs disrupt the functional integrity of the genome and possibly exhibit a genotoxic effect. |
| Databáze: | OpenAIRE |
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