Investigation of genotoxic effects of rhododendron honey using three mammalian bioassays in vivo
| Autor: | Salih Tunç Kaya, Fulya Dilek Gökalp, Meral Kekecoglu, Pinar Goc Rasgele, Merve Kambur Acar |
|---|---|
| Přispěvatelé: | [Belirlenecek] |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Lipid-Peroxidation sperm abnormality Rhododendron Health Toxicology and Mutagenesis Sperm abnormality 010501 environmental sciences Biology Toxicology 01 natural sciences Grayanotoxin-Iii Antioxidants 03 medical and health sciences Mice 0302 clinical medicine In vivo Bradycardia Bioassay Animals Humans 0105 earth and related environmental sciences Pharmacology Mammals Chemical Health and Safety Micronucleus Tests chromosomal aberration Public Health Environmental and Occupational Health General Medicine Honey Plants Mad honey Oxidative Stress Damage Biochemistry Seeds grayanatoxin Grayanotoxin Biological Assay micronucleus formation 030217 neurology & neurosurgery DNA Damage |
| Popis: | Rhododendron honey (RH) is obtained from the rhododendron plants are grown in many regions around the world, causes poisoning in humans due to the grayanotoxin (GTX) compound in its structure. It is used by the public as a therapeutic for some diseases. It was aimed to study the genotoxic and cytotoxic effects of RH in mouse bone-marrow and sperm cells by using three mammalian bioassays. 25, 50 and 75 mg kg(-1) concentrations of RH given to male mice via gavage for 24 and 48 h treatment periods and its active ingredient Grayanatoxin (GTX-III) 0.01 mg kg(-1) by i.p. injection. Chromosome aberrations (CA), polychromatic erythrocytes (PCE)/normochromatic erythrocytes (NCE), micronucleated polychromatic erythrocytes (MNPCE) and sperm abnormalities were investigated. The results demonstrated that all the tested concentrations of RH significantly induced total abnormal cell frequency including chromosomal breaks for two time periods. In the MN assay, 75 mg kg(-1) RH and 0.01 mg kg(-1) GTX-III significantly increased % MNPCE and significantly reduced PCE/NCE ratios after 24 and 48 h treatments on mice demonstrating potential genotoxic and cytotoxic effect. Although there was a concentration-related increase in the percentage of total sperm abnormalities, this increase was not statistically significant compared to control. As a result, microscopic genotoxicity and cytotoxicity marker tests showed that RH and its active ingredient GTX-III have potential genotoxic and cytotoxic effect on mice bone marrow cells. It is understood that RH that is used to treat some diseases by public, should be handled carefully and used in a controlled manner. Duzce University Scientific Research FundDuzce University [2013.01.01.199] This study was supported by Duzce University Scientific Research Fund [Project Number= 2013.01.01.199]. WOS:000658928300001 2-s2.0-85107611857 PubMed: 34100323 |
| Databáze: | OpenAIRE |
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