Light-mediated Kleaf induction and contribution of both the PIP1s and PIP2s aquaporins in five tree species: walnut (Juglans regia) case study

Autor: Sadok Bouzid, Didier Combes, Amélie Rabot, Jean-Stéphane Venisse, Khaoula Ben Baaziz, David Lopez, Soulaiman Sakr, Hervé Cochard, Aurelie Gousset
Přispěvatelé: Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ministere de l'Enseignement Superieur et de la Recherche, Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Rok vydání: 2012
Předmět:
0106 biological sciences
hydraulic conductance
Salicaceae
Transcription
Genetic
Light
Physiology
leaf hydraulic conductance
Gene Expression
Plant Science
Fagaceae
01 natural sciences
cell water relations
Quercus
Gene expression
Fagus
Arabidopsis thaliana
[SDV.SA.HORT]Life Sciences [q-bio]/Agricultural sciences/Horticulture
Plant Proteins
arbre
0303 health sciences
Vegetal Biology
drought stress
plasma-membrane aquaporins
trees
Hydraulic conductance
AQUAPORIN GENE EXPRESSION/LEAF HYDRAULIC CONDUCTANCE/LIGHT/TREES
rain-forest trees
Tree species
Juglans
functional-analysis
juglans regia
Aquaporin
arabidopsis-thaliana
Environment
Biology
Aquaporins
Genes
Plant
Quercus robur
03 medical and health sciences
Fagus sylvatica
Botany
FORESTRY
030304 developmental biology
quercus-macrocarpa leaves
lumière
Plant Transpiration
15. Life on land
biology.organism_classification
gene-expression
aquaporin gene expression
Plant Leaves
parenchyma cells
Biologie végétale
010606 plant biology & botany
Zdroj: Tree Physiology (Oxford Academic) 4 (32), 423-434. (2012)
Tree Physiology
Tree Physiology, Oxford University Press (OUP): Policy B-Oxford Open Option B, 2012, 32 (4), pp.423-434. ⟨10.1093/treephys/tps022⟩
Tree Physiology, 2012, 32 (4), pp.423-434. ⟨10.1093/treephys/tps022⟩
ISSN: 1758-4469
0829-318X
Popis: Understanding the response of leaf hydraulic conductance (Kleaf) to light is a challenge in elucidating plant–water relationships. Recent data have shown that the effect of light on Kleaf is not systematically related to aquaporin regulation, leading to conflicting conclusions. Here we investigated the relationship between light, Kleaf, and aquaporin transcript levels in five tree species (Juglans regia L., Fagus sylvatica L., Quercus robur L., Salix alba L. and Populus tremula L.) grown in the same environmental conditions, but differing in their Kleaf responses to light. Moreover, the Kleaf was measured by two independent methods (high-pressure flow metre (HPFM) and evaporative flux method (EFM)) in the most (J. regia) and least (S. alba) responsive species and the transcript levels of aquaporins were analyzed in perfused and unperfused leaves. Here, we found that the light-induced Kleaf value was closely related to stronger expression of both the PIP1 and PIP2 aquaporin genes in walnut (J. regia), but to stimulation of PIP1 aquaporins alone in F. sylvatica and Q. robur. In walnut, all newly identified aquaporins were found to be upregulated in the light and downregulated in the dark, further supporting the relationship between the light-mediated induction of Kleaf and aquaporin expression in walnut. We also demonstrated that the Kleaf response to light was quality-dependent, Kleaf being 60% lower in the absence of blue light. This decrease in Kleaf was correlated with strong downregulation of three PIP2 aquaporins and of all the PIP1 aquaporins tested. These data support a relationship between light-mediated Kleaf regulation and the abundance of aquaporin transcripts in the walnut tree.
Databáze: OpenAIRE
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