Genome-Wide Identification and Expression Analysis of the Ascorbate Oxidase Gene Family in Gossypium hirsutum Reveals the Critical Role of GhAO1A in Delaying Dark-Induced Leaf Senescence
| Autor: | Xiang Jin, Lihua Chen, Hongbin Li, Fei Wang, Shuangquan Xie, Aiping Cao, Xifeng Chen, Wangyang Song, Ze Pan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Senescence leaf senescence Transgene H2O2 Mutant Gossypium hirsutum Biology 01 natural sciences Catalysis Article Inorganic Chemistry 03 medical and health sciences Gene Expression Regulation Plant Arabidopsis Gene family Physical and Theoretical Chemistry Molecular Biology Gene light responsiveness Spectroscopy Plant Proteins Gossypium Organic Chemistry General Medicine Hydrogen Peroxide Darkness Ascorbic acid biology.organism_classification Plants Genetically Modified Phenotype Computer Science Applications Cell biology Plant Leaves 030104 developmental biology Ascorbate Oxidase 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 24 |
| ISSN: | 1422-0067 |
| Popis: | Ascorbate oxidase (AO) plays important roles in plant growth and development. Previously, we reported a cotton AO gene that acts as a positive factor in cell growth. Investigations on Gossypium hirsutum AO (GhAO) family genes and their multiple functions are limited. The present study identified eight GhAO family genes and performed bioinformatic analyses. Expression analyses of the tissue specificity and developmental feature of GhAOs displayed their diverse expression patterns. Interestingly, GhAO1A demonstrated the most rapid significant increase in expression after 1 h of light recovery from the dark. Additionally, the transgenic ao1-1/GhAO1A Arabidopsis lines overexpressing GhAO1A in the Arabidopsis ao1-1 late-flowering mutant displayed a recovery to the normal phenotype of wild-type plants. Moreover, compared to the ao1-1 mutant, the ao1-1/GhAO1A transgenic Arabidopsis presented delayed leaf senescence that was induced by the dark, indicating increased sensitivity to hydrogen peroxide (H2O2) under normal conditions that might be caused by a reduction in ascorbic acid (AsA) and ascorbic acid/dehydroascorbate (AsA/DHA) ratio. The results suggested that GhAOs are functionally diverse in plant development and play a critical role in light responsiveness. Our study serves as a foundation for understanding the AO gene family in cotton and elucidating the regulatory mechanism of GhAO1A in delaying dark-induced leaf senescence. |
| Databáze: | OpenAIRE |
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