Novel In Silico Analyses of Repetitive Spider Silk Sequences to Understand the Evolution and Mechanical Properties of Fibrous Protein Materials
| Autor: | Yun Jung Yang, Hyung Joon Cha, Dooyup Jung |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Globular protein In silico Silk Trimer Computational biology Biology complex mixtures 01 natural sciences Applied Microbiology and Biotechnology Arthropod Proteins Evolution Molecular Tandem repeat 010608 biotechnology Animals Spider silk Computer Simulation Gene Phylogeny Repetitive Sequences Nucleic Acid chemistry.chemical_classification Spider fungi 010401 analytical chemistry technology industry and agriculture Spiders General Medicine Sequence Analysis DNA equipment and supplies 0104 chemical sciences Biomechanical Phenomena SILK chemistry Molecular Medicine |
| Zdroj: | Biotechnology journal. 14(10) |
| ISSN: | 1860-7314 |
| Popis: | The mechanical properties of spider silks have diverged as spiders have diversely speciated. Because the main components of silks are proteins, it is valuable to investigate their sequences. However, silk sequences have been regarded as difficult information to analyze due to their imbalance and imperfect tandem repeats (ITR). Here, an in silico approach is applied to systemically analyze a group of silk sequences. It is found that every time new spider groups emerge, unique trimer motifs appear. These trimer motifs are used to find additional clues of evolution and to determine relationships with mechanical properties. For the first time, crucial evidence is provided that shows silk sequences coevolved with spider species and the mechanical properties of their fibers to adapt to new living environments. This novel approach can be used as a platform for analyzing other groups of ITR-harboring proteins and to obtain information for the design of tailor-made fibrous protein materials. |
| Databáze: | OpenAIRE |
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