Comparative Transcriptome Analysis of Streptomyces Clavuligerus in Response to Favorable and Restrictive Nutritional Conditions
| Autor: | León F. Toro, Emma Laing, Laura Pinilla, Juan F. Alzate, Rigoberto Ríos-Estepa |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Proteases 030106 microbiology Streptomyces clavuligerus gene cluster Biochemistry Microbiology Article Transcriptome 03 medical and health sciences complex media Gene cluster Gene expression Pharmacology (medical) clavulanic acid General Pharmacology Toxicology and Pharmaceutics Gene transcriptomic analysis Genetics biology lcsh:RM1-950 Genetic strain biology.organism_classification Chemically defined medium lcsh:Therapeutics. Pharmacology 030104 developmental biology Infectious Diseases gene expression |
| Zdroj: | Antibiotics Antibiotics, Vol 8, Iss 3, p 96 (2019) Volume 8 Issue 3 |
| ISSN: | 2079-6382 |
| DOI: | 10.3390/antibiotics8030096 |
| Popis: | Background: Clavulanic acid (CA), a &beta lactamase inhibitor, is industrially produced by the fermentation of Streptomyces clavuligerus. The efficiency of CA production is associated with media composition, culture conditions and physiological and genetic strain characteristics. However, the molecular pathways that govern CA regulation in S. clavuligerus remain unknown. Methods and Results: Here we used RNA-seq to perform a comparative transcriptome analysis of S. clavuligerus ATCC 27064 wild-type strain grown in both a favorable soybean-based medium and in limited media conditions to further contribute to the understanding of S. clavuligerus metabolism and its regulation. A total of 350 genes were found to be differentially expressed between conditions 245 genes were up-regulated in favorable conditions compared to unfavorable. Conclusion: The up-regulated expression of many regulatory and biosynthetic CA genes was positively associated with the favorable complex media condition along with pleiotropic regulators, including proteases and some genes whose biological function have not been previously reported. Knowledge from differences between transcriptomes from complex/defined media represents an advance in the understanding of regulatory paths involved in S. clavuligerus&rsquo metabolic response, enabling the rational design of future experiments. |
| Databáze: | OpenAIRE |
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