Colorimetric detection of ciprofloxacin in aqueous solution based on an unmodified aptamer and the aggregation of gold nanoparticles
| Autor: | Lumei Wang, Yichen Wang, Chaoqiang Pan, Guoqing Shen, Weiwen Huang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
General Chemical Engineering
Aptamer Metal Nanoparticles 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Colorimetry (chemical method) Analytical Chemistry chemistry.chemical_compound Ciprofloxacin Metal nanoparticles Detection limit Aqueous solution General Engineering Aptamers Nucleotide 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Colloidal gold Ammonium chloride Colorimetry Gold Absorption (chemistry) 0210 nano-technology Nuclear chemistry |
| Zdroj: | Analytical methods : advancing methods and applications. 13(1) |
| ISSN: | 1759-9679 |
| Popis: | A colorimetric method is described for detection of the antibiotic ciprofloxacin (CIP) in aqueous solutions based on an unmodified CIP-aptamer and gold nanoparticles (AuNPs), which are regarded as a probe and an indicator, respectively. In the absence of CIP, aptamers can hybridize with poly dimethyl diallyl ammonium chloride (PDDA) to form a 'duplex' structure through electrostatic interactions, and AuNPs remain dispersed in solution, leading to a red solution and an obvious absorption peak at 520 nm. Contrarily, the CIP-aptamer can specifically bind to CIP after the introduction of CIP into the solution. Then PDDA is thus free to aggregate AuNPs. The solution turns blue from red accordingly, presenting a strong absorption at 650 nm. Hence, the concentration of CIP can be quantified through the changes of the absorption. This sensitive and selective colorimetric method for CIP detection has a good linear response (R = 0.9935) in the 20 to 300 nM CIP concentration range, with a limit of detection (LOD) of 0.215 nM. Importantly, the proposed aptasensor demonstrates great application potential in CIP determination in aqueous samples. |
| Databáze: | OpenAIRE |
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