A case of failed oocyte maturation in vivo and in vitro
| Autor: | Lucas Klentzeris, Susan Montgomery, Geraldine Hartshorne |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Infertility endocrine system Germinal vesicle medicine.medical_treatment Cell Cycle Obstetrics and Gynecology Fertilization in Vitro Biology medicine.disease Oocyte Oogenesis Intracytoplasmic sperm injection Chromatin Andrology medicine.anatomical_structure Reproductive Medicine Oocytes medicine Humans Female Mitosis Gonadotropins Unexplained infertility |
| Zdroj: | Fertility and Sterility. 71:567-570 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(98)00505-6 |
| Popis: | Objective: To study a couple for whom all oocytes failed to mature during three attempts at IVF/ICSI treatment. Our aims were (a) to promote oocyte maturation in vitro and (b) to identify the stage of maturation arrest. Design: Case report. Setting: Infertility treatment center with academic research links. Patient(s): Couple with unexplained infertility. Intervention(s): Exogenous gonadotropin stimulation culminating in the retrieval of eight oocyte-cumulus complexes that were exposed to gonadotropins for 24 hours in vitro. Main Outcome Measure(s): Observation of oocyte cumulus complexes, measurements of oocyte diameter, oocyte chromatin staining with Hoechst 33258. Result(s): The cumulus complexes varied in appearance and expanded in vitro, but yielded immature oocytes. Germinal vesicle breakdown occurred in all oocytes and chromatin was consistent with arrest at entry into M-phase. The oocytes were of a size normally competent to mature. Conclusion(s): Infertility in this couple was attributed to the failure of oocytes to enter M-phase. This was not overcome by exposure to gonadotropins in vitro nor associated with incompetence due to small oocyte size. |
| Databáze: | OpenAIRE |
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