Uptake and Incorporation of Glucose and Mannose by Whole Cells of Bacteroides thetaiotaomicron
| Autor: | Phillip B. Hylemon, R. F. Roadcap, P. V. Phibbs, J. L. Young |
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| Rok vydání: | 1977 |
| Předmět: |
Azides
Dicumarol Vitamin K Phosphofructokinase-1 Glucose uptake Mannose Glucosephosphate Dehydrogenase Applied Microbiology and Biotechnology Fluorides chemistry.chemical_compound Fumarates Fructose-Bisphosphate Aldolase Hexokinase Bacteroides Anaerobiosis Lactose Cyanides Ecology biology Phosphogluconate Dehydrogenase Polysaccharides Bacterial Glucose-6-Phosphate Isomerase Fructose biology.organism_classification Aerobiosis Glucose chemistry Biochemistry Galactose Hydroxyquinolines Chloromercuribenzoates Bacteroides thetaiotaomicron Dinitrophenols Metabolism Growth and Industrial Microbiology Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 34:488-494 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.34.5.488-494.1977 |
| Popis: | Glucose uptake by whole-cell suspensions of the obligate anaerobe Bacteroides thetaiotaomicron was two- to fourfold higher under aerobic conditions than during incubation under atmospheres of N 2 or H 2 gas. The O 2 -stimulated uptake activity was lost rapidly (>70% in 5 h) when cell suspensions were incubated aerobically, but this loss was prevented by the addition of crude catalase. Catalase had no apparent effect on cell viability during these incubations. Glucose uptake activity was strongly inhibited by a 10-fold excess of mannose or galactose but not by methyl-α- d -glucoside, fructose, or lactose. Both glucose and mannose were rapidly incorporated into polyglucose after uptake. The O 2 -stimulated glucose uptake was not inhibited by cyanide, azide, 2,4-dinitrophenol, or 2- N -heptyl-4-hydroxyquinoline- N -oxide. However, p -chloromercuribenzoate, menadione, and sodium fluoride inhibited uptake by 88, 67, and 55%, respectively. All attempts to detect phosphoenolpyruvate-phosphotransferase activity for glucose, methyl-α- d -glucoside, and 2-deoxyglucose were negative. The bacteria contained hexokinase activity and a complete glycolytic Embden-Meyerhof pathway. |
| Databáze: | OpenAIRE |
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