LncRNA NEAT1 promotes glioma cancer progression via regulation of miR-98-5p/BZW1
| Autor: | Xi-rui Wang, Gang Li, Yabin Li, Zhihuang Zhao, Ruijian Zhang, Jinxing Shang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
NEAT1 Biophysics Mice Nude Cell Cycle Proteins Biology medicine.disease_cause Biochemistry 03 medical and health sciences 0302 clinical medicine In vivo Cell Line Tumor Glioma medicine Animals Humans Molecular Biology Research Articles Cancer Cell Proliferation Mice Inbred BALB C Gene knockdown Brain Neoplasms Cell growth Paraspeckle BZW1 Cell Biology medicine.disease Tumor Burden DNA-Binding Proteins Gene Expression Regulation Neoplastic Blot miR-98-5p MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Disease Progression Cancer research Female RNA Long Noncoding Carcinogenesis Signal Transduction |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| DOI: | 10.1042/bsr20200767 |
| Popis: | Background: Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas.Methods: Quantitative real-time PCR technology was used to determine the expression levels of relevant genes in tumor tissues and cell lines. The protein expression levels were validated by Western blotting. Cell counting kit-8 (CCK-8) and colony formation assays were used to test the cell proliferation ability. A luciferase reporter assay was used to determine the interactions of the genes. Tumor xenografts were used to detect the role of NEAT1 in gliomas in vivo.Results: We demonstrated that NEAT1 up-regulated glioma cells and negatively correlated with miR-98-5p in glioma tissues. A potential binding region between NEAT1 and miR-98-5p was confirmed by dual-luciferase assays. NEAT1 knockdown inhibited glioma cell proliferation. The inhibition of miR-98-5p rescued the knockdown of NEAT1 in glioma cells. Basic leucine zipper and W2 domain containing protein 1 (BZW1) was identified as a direct target of miR-98-5p. We also identified that BZW1 was positively correlated with NEAT1 in glioma tissues. NEAT1 knockdown inhibited glioma cell proliferation in vivo via miR-98-5p/BZW1.Conclusion: Our results suggest that NEAT1 plays an oncogenic function in glioma progression. Targeting NEAT1/miR-98-5p/BZW1 may be a novel therapeutic treatment approach for glioma patients. |
| Databáze: | OpenAIRE |
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