Evaluation of a recombinant measles virus expressing hepatitis C virus envelope proteins by infection of human PBL-NOD/Scid/Jak3null mouse
| Autor: | Salem Nagla Elwy Salem Ali, Seiji Okada, Sumako Iwanaga, Makoto Saito, Michinori Kohara, Kyoko Tsukiyama-Kohara, Shinji Harada, Takahiro Seki, Kohsuke Tanaka, Chieko Kai, Masaaki Satoh |
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| Rok vydání: | 2010 |
| Předmět: |
Viral Hepatitis Vaccines
Hepatitis C virus viruses Immunology Blotting Western Spleen Nod Mice SCID Biology medicine.disease_cause Microbiology Polymerase Chain Reaction Virus Article Cell Line Measles virus E1 Mice Cytopathogenic Effect Viral Viral Envelope Proteins E2 Splenocyte medicine Immunology and Allergy Animals Humans Human PBL Vaccines Synthetic General Veterinary Immunogenicity Callithrix General Medicine biology.organism_classification Flow Cytometry Virology Hepatitis C Coculture Techniques NOD/Scid/Jak3null mouse Infectious Diseases medicine.anatomical_structure HCV biology.protein Leukocytes Mononuclear MV Antibody |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases |
| ISSN: | 1878-1667 |
| Popis: | In this study, we infected NOD/Scid/Jak3null mice engrafted human peripheral blood leukocytes (hu-PBL-NOJ) with measles virus Edmonston B strain (MV-Edm) expressing hepatitis C virus (HCV) envelope proteins (rMV-E1E2) to evaluate the immunogenicity as a vaccine candidate. Although human leukocytes could be isolated from the spleen of mock-infected mice during the 2-weeks experiment, the proportion of engrafted human leukocytes in mice infected with MV (10 3 –10 5 pfu) or rMV-E1E2 (10 4 pfu) was decreased. Viral infection of the splenocytes was confirmed by the development of cytopathic effects (CPEs) in co-cultures of splenocytes and B95a cells and verified using RT-PCR. Finally, human antibodies against MV were more frequently observed than E2-specific antibodies in serum from mice infected with a low dose of virus (MV, 10 0 –10 1 pfu, and rMV-E1E2, 10 1 –10 2 pfu). These results showed the possibility of hu-PBL-NOJ mice for the evaluation of the immunogenicity of viral proteins. |
| Databáze: | OpenAIRE |
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