Increased phenolic acid and tanshinone production and transcriptional responses of biosynthetic genes in hairy root cultures of Salvia przewalskii Maxim. treated with methyl jasmonate and salicylic acid
| Autor: | Jing Gao, Yang Bingyue, Lu Luo, Jie Li, Yonggang Yan, Bo Li, Hu Benxiang, Fulin Cao, Gang Zhang, Peng Liang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Time Factors Cyclopentanes Acetates Depsides Plant Roots 03 medical and health sciences chemistry.chemical_compound Caffeic Acids 0302 clinical medicine Plant Growth Regulators Gene Expression Regulation Plant Gene expression Hydroxybenzoates Genetics Caffeic acid Oxylipins Salvia Salvia przewalskii Molecular Biology Benzofurans Plant Proteins Methyl jasmonate Dose-Response Relationship Drug biology Chemistry Rosmarinic acid General Medicine Phenolic acid Phenanthrenes biology.organism_classification 030104 developmental biology Biochemistry Cinnamates 030220 oncology & carcinogenesis Abietanes Plant hormone Salicylic Acid Salicylic acid |
| Zdroj: | Molecular Biology Reports. 47:8565-8578 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-020-05899-1 |
| Popis: | The purpose of this study is to reveal the impact of the plant hormone salicylic acid (SA) and methyl jasmonate (MeJA) on the growth, effective components accumulation, and related gene expression of the hairy root of Salvia przewalskii Maxim. Various concentrations of SA (0, 25, 50, 100, 200 μM) or MeJA (0, 50, 100, 200, 400, 600 μM) were added to the culture medium of Salvia przewalskii Maxim. Low concentrations of SA promoted the growth of hairy root, while a high concentration inhibited it. 0 to 400 μM MeJA promoted the growth of hairy root, but 600 μM MeJA starts to inhibit its growth. 50 μM SA and 400 μM MeJA significantly enhanced the production of caffeic acid, rosmarinic acid, salvianolic acid B, cryptotanshinone, and tanshinone IIA. In general, 50 μM SA can be used to accumulate of tanshinone in hairy roots of S. przewalskii with 6 days. 400 μM MeJA can be used to accumulate of phenolic acids in hairy roots of S. przewalskii with 3 days. The selected genes in the tanshinone and phenolic acid biosynthetic pathway were upregulated with elicitation. To obtain a higher yield and content of secondary metabolites, it is advisable to use 50 μM SA or 400 μM MeJA as the optimal doses to cultivate the hairy root of S. przewalskii. This study provides, for the first time, an efficient tanshinone and phenolic acid production method for S. przewalskii. |
| Databáze: | OpenAIRE |
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