Adherent-Invasive E. coli enhances colonic hypersensitivity and P2X receptors expression during post-infectious period
| Autor: | Frederic A. Carvalho, Agathe Gelot, Nicolas Barnich, Amandine Lashermes, Ludivine Boudieu, Julie Barbier, Benoit Sion, Denis Ardid |
|---|---|
| Přispěvatelé: | Neuro-Dol (Neuro-Dol), Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'endocrinologie moléculaire, Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Pharmacologie fondamentale et clinique de la douleur, Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION ), Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine MESH: Inflammation MESH: Receptors Purinergic P2X Inflammatory bowel disease Mice Adherent invasive E. coli 0302 clinical medicine Crohn Disease MESH: Colitis MESH: Up-Regulation MESH: Animals Intestinal Mucosa Receptor MESH: Antigens CD Escherichia coli Infections Irritable bowel syndrome 2. Zero hunger colonic hypersensitivity MESH: Escherichia coli Gastroenterology Colitis Up-Regulation 3. Good health Infectious Diseases medicine.anatomical_structure Receptors Purinergic P2X MESH: Permeability MESH: Intestinal Mucosa MESH: Cell Adhesion Molecules Female 030211 gastroenterology & hepatology visceral pain medicine.symptom Microbiology (medical) Genetically modified mouse Colon MESH: Mice Transgenic Mice Transgenic Inflammation Ileum GPI-Linked Proteins Microbiology Permeability 03 medical and health sciences Antigens CD MESH: Mice Inbred C57BL Escherichia coli medicine Animals MESH: Colon MESH: Mice MESH: Escherichia coli Infections Intestinal permeability MESH: Crohn Disease business.industry intestinal permeability P2X receptors Visceral pain [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology medicine.disease [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Male Mice Inbred C57BL 030104 developmental biology MESH: Ileum Research Paper/Report Immunology MESH: GPI-Linked Proteins business Cell Adhesion Molecules MESH: Female |
| Zdroj: | Gut microbes Gut microbes, Taylor & Francis, 2017, 9 (1), pp.26-37. ⟨10.1080/19490976.2017.1361091⟩ Gut microbes, 2017, 9 (1), pp.26-37. ⟨10.1080/19490976.2017.1361091⟩ |
| ISSN: | 1949-0976 1949-0984 |
| Popis: | International audience; Irritable Bowel Syndrome (IBS) and Inflammatory Bowel Disease (IBD) are related gastrointestinal disorders characterized by abdominal pain associated with colonic hypersensitivity (CHS). Studies in humans have reported an abnormal colonization of Adherent-Invasive E. coli (AIEC) in the ileum of Crohn's disease (CD) patients associated with overexpression of the bacterial colonizing receptor CEACAM6. The aim of the present study was to investigate whether AIEC reference strain LF82 could induce intestinal impairment during infectious and/or post-infectious periods and subsequently the development of CHS. Transgenic mice overexpressing human CEACAM6 protein (TG) and their wild-type littermates were gavaged by CD-associated AIEC bacteria (reference strain LF82) or PBS for 3 d. Colonic hypersensitivity was assessed by colorectal distension (CRD) test during infectious (D4) and post-infectious periods (D21). Several markers of intestinal inflammation were monitored and the colonic expression of purinergic P2X receptors was quantified. At D4, an increased visceromotor response (VMR) to the CRD test was observed in TG mice infected with CD-associated AIEC LF82 in comparison with non-infected TG mice and persisted in a subgroup of infected animals at D21 after bacteria clearance. Increased VMR was associated with low-grade intestinal inflammation, increased intestinal permeability and expression of P2X 3, 4 and 7. This study shows that certain susceptible hosts infected with CD-associated AIEC bacteria can develop persistent CHS associated with low-grade inflammation and increased P2X receptors expression. Thus, CD-associated AIEC infection in CEACAM6 transgenic mice could be used as a novel post-infectious mouse model mimicking quiescent IBD with IBS-like symptoms such as visceral pain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|