Ross River Virus Envelope Glycans Contribute to Type I Interferon Production in Myeloid Dendritic Cells
| Autor: | Kristin M. Rogers, Reed S. Shabman, Mark T. Heise |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Glycosylation
viruses Immunology Genome Viral Alphavirus Biology Microbiology Virus Cell Line Ross River virus chemistry.chemical_compound Viral Envelope Proteins Viral envelope Aedes Polysaccharides Interferon Cricetinae Virology medicine Animals Myeloid Cells Dendritic Cells biology.organism_classification Type I interferon production chemistry Insect Science Interferon Type I Mutation Togaviridae Pathogenesis and Immunity medicine.drug |
| Zdroj: | Journal of Virology. 82:12374-12383 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.00985-08 |
| Popis: | Alphaviruses are mosquito-transmitted viruses that cause significant human disease, and understanding how these pathogens successfully transition from the mosquito vector to the vertebrate host is an important area of research. Previous studies demonstrated that mosquito and mammalian-cell-derived alphaviruses differentially induce type I interferons (alpha/beta interferon [IFN-α/β]) in myeloid dendritic cells (mDCs), where the mosquito cell-derived virus is a poor inducer of IFN-α/β compared to the mammalian-cell-derived virus. Furthermore, the reduced IFN-α/β induction by the mosquito cell-derived virus is attributed to differential N-linked glycosylation (29). To further evaluate the role of viral envelope glycans in regulating the IFN-α/β response, studies were performed to assess whether the mosquito cell-derived virus actively inhibits IFN-α/β induction or is simply a poor inducer of IFN-α/β. Coinfection studies using mammalian- and mosquito cell-derived Ross River virus (mam-RRV and mos-RRV, respectively) indicated that mos-RRV was unable to suppress IFN-α/β induction by mam-RRV in mDC cultures. Additionally, a panel of mutant viruses lacking either individual or multiple N-linked glycosylation sites was used to demonstrate that N-linked glycans were essential for high-level IFN-α/β induction by the mammalian-cell-derived virus. These results suggest that the failure of the mosquito cell-derived virus to induce IFN-α/β is due to a lack of complex carbohydrates on the virion rather than the active suppression of the DC antiviral response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|