Effect of dehydroepiandrosterone on pentose phosphate pathway activity in the rat colon
| Autor: | V. Pascoe, D. Antoniou, W.J. Butler, R.N. Butler |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Colon medicine.medical_treatment Intraperitoneal injection Dehydroepiandrosterone Butyrate Biology Pentose phosphate pathway Biochemistry Pentose Phosphate Pathway Rats Sprague-Dawley chemistry.chemical_compound Internal medicine Ribose medicine Animals Hexose chemistry.chemical_classification Metabolism Carbohydrate Rats Endocrinology chemistry Starvation Female Oxidation-Reduction |
| Zdroj: | International Journal of Biochemistry. 25:1601-1607 |
| ISSN: | 0020-711X |
| DOI: | 10.1016/0020-711x(93)90518-j |
| Popis: | 1. 1. The effects of fasting and fasting followed by refeeding on the activities of the oxidative pentose pathway (OPP) and the non-oxidative pentose pathway (NOPP) were estimated by the rate of production of 14CO2 from [l-14C] glucose in isolated rat colonocytes, and the production of hexose 6-phosphates from ribose 5-phosphate in rat colonic cytosols, respectively. 2. 2. The OPP activity in colonocytes from rats in the fasted state was 50% lower when compared to colonocytes from rats refed after a fast. This indicated induction of the rate-limiting enzyme of the OPP, glucose 6-P dehydrogenase (G6-PDH) in the latter instance. No effect on the maximal catalytic activity of the enzymes of the NOPP was seen in colonocytes from rats refed after a fast compared with colonocytes from rats in the fasted state. 3. 3. Isolated colonocytes obtained from the distal colon of rats refed after a fast, showed a significant decrease (30%) in OPP activity when incubated with 50 μ M dehydroepiandrosterone (DHEA). A similar degree of inhibition was seen with 10 mM butyrate (P |
| Databáze: | OpenAIRE |
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