Meiotic chromosomal core consisting of cohesin complex proteins recruits DNA recombination proteins and promotes synapsis in the absence of an axial element in mammalian meiotic cells
| Autor: | Eva Brundell, Peter B. Moens, Christer Höög, Rolf Jessberger, Jian-Guo Liu, Christa Heyting, Li Yuan, José Luis Barbero, Sabine Santucci-Darmanin, Jeanette Pelttari, Mary-Rose Hoja |
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| Přispěvatelé: | UMR 6549 CNRS, Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Cohesin complex
Chromosomal Proteins Non-Histone [SDV]Life Sciences [q-bio] Cell Cycle Proteins Synaptonemal complex protein 3 Biology Chromosomes Chromosomal crossover Fungal Proteins Chromosome segregation 03 medical and health sciences Meiosis Animals Life Science Molecular Biology 030304 developmental biology Genetics 0303 health sciences Cohesin 030302 biochemistry & molecular biology Synapsis Nuclear Proteins Cell Biology DNA Dynamics and Chromosome Structure Recombinant Proteins DNA-Binding Proteins Synaptonemal complex COS Cells Synapses EPS Wageningen University |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2001, 21 (16), pp.5667-5677. ⟨10.1128/mcb.21.16.5667-5677.2001⟩ Molecular and Cellular Biology 22 (2001) Molecular and Cellular Biology, 22, 5667-5677 |
| ISSN: | 0270-7306 1098-5549 |
| DOI: | 10.1128/mcb.21.16.5667-5677.2001 |
| Popis: | International audience; ABSTRACT The behavior of meiotic chromosomes differs in several respects from that of their mitotic counterparts, resulting in the generation of genetically distinct haploid cells. This has been attributed in part to a meiosis-specific chromatin-associated protein structure, the synaptonemal complex. This complex consist of two parallel axial elements, each one associated with a pair of sister chromatids, and a transverse filament located between the synapsed homologous chromosomes. Recently, a different protein structure, the cohesin complex, was shown to be associated with meiotic chromosomes and to be required for chromosome segregation. To explore the functions of the two different protein structures, the synaptonemal complex and the cohesin complex, in mammalian male meiotic cells, we have analyzed how absence of the axial element affects early meiotic chromosome behavior. We find that the synaptonemal complex protein 3 (SCP3) is a main determinant of axial-element assembly and is required for attachment of this structure to meiotic chromosomes, whereas SCP2 helps shape the in vivo structure of the axial element. We also show that formation of a cohesin-containing chromosomal core in meiotic nuclei does not require SCP3 or SCP2. Our results also suggest that the cohesin core recruits recombination proteins and promotes synapsis between homologous chromosomes in the absence of an axial element. A model for early meiotic chromosome pairing and synapsis is proposed. |
| Databáze: | OpenAIRE |
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