T-, B- and NK-lymphoid, but not myeloid cells arise from human CD34(+)CD38(-)CD7(+) common lymphoid progenitors expressing lymphoid-specific genes
| Autor: | Georges Leclercq, Inge Hoebeke, Karin Pike-Overzet, Frank J. T. Staal, F Stolz, M De Smedt, Jean Plum |
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| Přispěvatelé: | Immunology |
| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
Myeloid T-Lymphocytes Cellular differentiation Cell Culture Techniques Antigens CD34 Antigens CD7 Biology Models Biological Antigens CD hemic and lymphatic diseases medicine Humans Lymphopoiesis Interleukin 3 B-Lymphocytes Infant Newborn Cell Differentiation Hematology Fetal Blood Hematopoietic Stem Cells Natural killer T cell ADP-ribosyl Cyclase 1 Molecular biology Coculture Techniques Hematopoiesis Killer Cells Natural Haematopoiesis medicine.anatomical_structure Oncology Immunology Bone marrow Stem cell |
| Zdroj: | Leukemia, 21(2), 311-319. Nature Publishing Group |
| ISSN: | 0887-6924 |
| DOI: | 10.1038/sj.leu.2404488 |
| Popis: | Hematopoietic stem cells in the bone marrow (BM) give rise to all blood cells. According to the classic model of hematopoiesis, the differentiation paths leading to the myeloid and lymphoid lineages segregate early. A candidate 'common lymphoid progenitor' (CLP) has been isolated from CD34(+)CD38(-) human cord blood cells based on CD7 expression. Here, we confirm the B- and NK-differentiation potential of CD34(+)CD38(-)CD7(+) cells and show in addition that this population has strong capacity to differentiate into T cells. As CD34(+)CD38(-)CD7(+) cells are virtually devoid of myeloid differentiation potential, these cells represent true CLPs. To unravel the molecular mechanisms underlying lymphoid commitment, we performed genome-wide gene expression profiling on sorted CD34(+)CD38(-)CD7(+) and CD34(+)CD38(-)CD7(-) cells. Interestingly, lymphoid-affiliated genes were mainly upregulated in the CD7(+) population, while myeloid-specific genes were downregulated. This supports the hypothesis that lineage commitment is accompanied by the shutdown of inappropriate gene expression and the upregulation of lineage-specific genes. In addition, we identified several highly expressed genes that have not been described in hematopoiesis before. |
| Databáze: | OpenAIRE |
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