E2F coregulates an essential HSF developmental program that is distinct from the heat-shock response
| Autor: | Richard I. Morimoto, Laetitia Chauve, Renée M. Brielmann, Grace Phelps, Jian Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Helminth genetics Biology 03 medical and health sciences Genetics Transcriptional regulation Animals Gene Regulatory Networks Heat shock Nucleotide Motifs E2F Caenorhabditis elegans Caenorhabditis elegans Proteins Promoter Regions Genetic Gene Genome Helminth Promoter biology.organism_classification Cell biology E2F Transcription Factors 030104 developmental biology Larva Biogenesis Heat-Shock Response Developmental Biology Research Paper Protein Binding |
| Popis: | Heat-shock factor (HSF) is the master transcriptional regulator of the heat-shock response (HSR) and is essential for stress resilience. HSF is also required for metazoan development; however, its function and regulation in this process are poorly understood. Here, we characterize the genomic distribution and transcriptional activity of Caenorhabditis elegans HSF-1 during larval development and show that the developmental HSF-1 transcriptional program is distinct from the HSR. HSF-1 developmental activation requires binding of E2F/DP to a GC-rich motif that facilitates HSF-1 binding to a heat-shock element (HSE) that is degenerate from the consensus HSE sequence and adjacent to the E2F-binding site at promoters. In contrast, induction of the HSR is independent of these promoter elements or E2F/DP and instead requires a distinct set of tandem canonical HSEs. Together, E2F and HSF-1 directly regulate a gene network, including a specific subset of chaperones, to promote protein biogenesis and anabolic metabolism, which are essential in development. |
| Databáze: | OpenAIRE |
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