Aerodigestive dysbiosis in children with chronic cough

Autor: Patrick Malecha, Benjamin G. Wu, Jessica Erkman, Jeremiah Levine, Libia Moy, Bianca Kapoor, Leopoldo N. Segal, Mikhail Kazachkov, Kathryn Fitzgerald, Yonghua Li
Rok vydání: 2018
Předmět:
Male
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Respiratory System
Bronchoalveolar Lavage
Gastroenterology
03 medical and health sciences
Enteral Nutrition
0302 clinical medicine
RNA
Ribosomal
16S
030225 pediatrics
Internal medicine
Bronchoscopy
Prevotella
medicine
Humans
Prospective Studies
Respiratory system
Bronchitis
Child
Asthma
Inflammation
biology
medicine.diagnostic_test
business.industry
Microbiota
Bacterial Infections
respiratory system
biology.organism_classification
medicine.disease
respiratory tract diseases
Chronic cough
Cross-Sectional Studies
Bronchoalveolar lavage
Cough
030228 respiratory system
Aerodigestive Tract
Child
Preschool
Chronic Disease
Pediatrics
Perinatology and Child Health
Dysbiosis
Female
Nervous System Diseases
medicine.symptom
business
Airway
Bronchoalveolar Lavage Fluid
Zdroj: Pediatric Pulmonology. 53:1288-1298
ISSN: 8755-6863
DOI: 10.1002/ppul.24115
Popis: In pediatric patients with chronic cough, respiratory culture techniques commonly yield negative results. Studies using culture-independent methods have found a high relative abundance of oral microbes in the lower airways, suggesting that the topographical continuity, and dynamics of the intraluminal contents of the aerodigestive system likely influence the lower airway microbiota. We hypothesize that in subjects with chronic cough, clinical diagnosis will correlate with distinct microbial signatures detected using culture-independent methods. Study design and methods We enrolled 36 pediatric subjects with chronic cough in a cross-sectional study. Subjects were categorized into four clinical groups: asthma, bacterial bronchitis, neurologically impaired-orally fed, and neurologically impaired enterally fed. Samples from the aerodigestive tract were obtained through bronchoscopy and upper endoscopy. 16S rRNA gene sequencing compared the microbiota from bronchoalveolar lavage (BAL), tracheal, supraglottic, esophageal, gastric, and duodenal samples. Results We observed that the lower airway microbiota of asthma subjects had higher α diversity as compared with the other groups. β diversity analysis of BAL samples revealed significant differences between the groups. Among the taxonomic differences found, most differentially enriched taxa were upper airway organisms such as Rothia, Gemellaceae (u.g. or uncharacterized genus), and Granulicatella in asthma, Prevotella in bacterial bronchitis, and Veillonella in neurologically impaired orally fed subjects. Greater dissimilarity between the upper airway and lower airway microbiota was associated with increased neutrophilic airway inflammation. Conclusions Distinct dysbiotic signatures can be identified in the lower airway microbiota of pediatric subjects with chronic cough that relates to the degree and type of inflammation.
Databáze: OpenAIRE
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