Characterization of inducible stx2-positive Escherichia coli O157:H7/H7- strains isolated from cattle in France

Autor: Jean-Yves Madec, Claire Maurer, Corinne Lazizzera
Přispěvatelé: Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes, Agence Française de Sécurité Sanitaire des Aliments (AFSSA)
Rok vydání: 2008
Předmět:
DNA/administration & dosage/genetics/immunology Vaccinia virus/*genetics/immunology Viral Vaccines/administration & dosage/genetics/immunology Virion/genetics/immunology Visna-maedi virusenrofloxacin
animal diseases
Antibiotics
env/*genetics/immunology Genes
medicine.disease_cause
Applied Microbiology and Biotechnology
Shiga Toxin 2
chemistry.chemical_compound
fluids and secretions
STX2
MESH: Cattle Diseases
MESH: Animals
O157:H7
Escherichia coli Infections
1UVIROLOGIE HAFSSA AUTRE Animals Antibodies
MESH: Microbial Sensitivity Tests
Enrofloxacin
biology
MESH: Enzyme-Linked Immunosorbent Assay
Shiga toxin
General Medicine
Enterobacteriaceae
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
STEC
MESH: Cattle
MESH: Electrophoresis
Gel
Pulsed-Field
MESH: Escherichia coli O157
Spectrophotometry
France
Viral/blood *Biolistics Epidermis/virology Female Genes
Biotechnology
medicine.drug
Fluoroquinolones
Progressive Interstitial
medicine.drug_class
Cattle Diseases
Enzyme-Linked Immunosorbent Assay
Microbial Sensitivity Tests
Escherichia coli O157
Microbiology
Shiga-like toxin
Species Specificity
MESH: Anti-Bacterial Agents
Escherichia coli
medicine
MESH: Species Specificity
Animals
MESH: Food Microbiology
MESH: Escherichia coli Infections
MESH: Spectrophotometry
MESH: Shiga Toxin 2
MESH: Fluoroquinolones
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Virology
MESH: France
chemistry
gag/*genetics/immunology Immunization Male Pneumonia
biology.protein
Food Microbiology
bacteria
Cattle
of Sheep/*prevention & control/virology Proviruses/isolation & purification Sheep T-Lymphocytes/immunology *Vaccines
Bacteria
Zdroj: Journal of Applied Microbiology
Journal of Applied Microbiology, Wiley, 2008, 104 (6), pp.1569-76. ⟨10.1111/j.1365-2672.2007.03692.x⟩
ISSN: 1365-2672
1364-5072
DOI: 10.1111/j.1365-2672.2007.03692.x⟩
Popis: Aims: To quantify the variability of the Shiga toxin 2 (Stx2) production by a panel of stx2-positive Escherichia coli O157:H7/H7- isolates from healthy cattle before and after induction with enrofloxacin. Methods and Results: ProSpecT® ELISA was used to quantify the Stx2 production by stx2-positive E. coli O157:H7/H7- isolates in native conditions (basal level) or after induction with enrofloxacin. Whereas only 15·2% of the E. coli O157:H7/H7- strains studied displayed significant amounts of detectable Stx2 without induction, most of them were shown to be inducible, and at various levels, in presence of subinhibitory concentrations of enrofloxacin. Conclusions: We demonstrated the capability of a highly elevated proportion of stx2-positive, but constitutively Stx2-negative, E. coli O157:H7/H7- isolates from healthy cattle to produce significant levels of Shiga toxin Stx2 in presence of subtherapeutic concentrations of enrofloxacin, an antibiotic of the fluoroquinolones family only licensed for veterinary use. Significance and Impact of the Study: This study documents the risk that bovine-associated Shiga toxin producing E. coli isolates may become more frequently pathogenic to humans as a side-effect of the increasing use of veterinary fluoroquinolones in the oral treatment of food animals like cattle or poultry.
Databáze: OpenAIRE
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