Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens
| Autor: | Shayan Sharif, John F. Prescott, Raveendra R. Kulkarni, Valeria R. Parreira |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Salmonella typhimurium
Salmonella animal structures Clostridium perfringens Pyruvate Synthase Salmonella Vaccines Administration Oral Vaccines Attenuated medicine.disease_cause Microbiology Enteritis Bacterial Proteins Antigen Fructose-Bisphosphate Aldolase medicine Animals Poultry Diseases Antigens Bacterial Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Body Weight Public Health Environmental and Occupational Health Salmonella vaccine biology.organism_classification medicine.disease Antibodies Bacterial Enterobacteriaceae Virology Intestines Infectious Diseases Salmonella enterica Clostridium Infections biology.protein Molecular Medicine Antibody Chickens |
| Zdroj: | Vaccine. 26:4194-4203 |
| ISSN: | 0264-410X |
| Popis: | Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens but currently no effective vaccine is available. Our previous study showed that certain C. perfringens secreted proteins when administered intramuscularly protected chickens against experimental infection. In the current study, genes encoding three C. perfringens proteins: fructose-biphosphate-aldolase (FBA), pyruvate:ferredoxin-oxidoreductase (PFOR) and hypothetical protein (HP), were cloned into an avirulent Salmonella enterica sv. typhimurium vaccine vector. Broiler chickens immunized orally with recombinant Salmonella expressing FBA or HP proteins were significantly protected against NE challenge. Immunized birds developed serum and mucosal antibodies to both clostridial and Salmonella antigens. This study showed the oral immunizing ability of two C. perfringens antigens against NE in broiler chickens through an attenuated Salmonella vaccine vector. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect