Evaluation of antibody response to an adjuvanted hapten-protein vaccine as a potential inhibitor of sexual maturation for farmed Atlantic salmon
| Autor: | Douglas C. Eckery, Lowell A. Miller, Ellen Marie Sætre, Jack C. Rhyan, Mo Salman, Christopher A. Myrick, Darcy S.O. Mora |
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| Rok vydání: | 2017 |
| Předmět: |
Fish Proteins
0301 basic medicine medicine.medical_specialty Vibrio anguillarum medicine.drug_class Salmo salar Peptide Aeromonas salmonicida Aquatic Science Immunostimulant Microbiology Random Allocation 03 medical and health sciences Adjuvants Immunologic Antigen Internal medicine medicine Animals Environmental Chemistry Vaccines Contraceptive Sexual Maturation Bovine serum albumin Vibrio chemistry.chemical_classification biology General Medicine Luteinizing Hormone biology.organism_classification 030104 developmental biology Endocrinology chemistry Antibody Formation Bacterial Vaccines biology.protein Antibody Haptens Hapten Keyhole limpet hemocyanin |
| Zdroj: | Fish & Shellfish Immunology. 71:255-263 |
| ISSN: | 1050-4648 |
| Popis: | An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated with one of four immunostimulants in a water-in-oil emulsion. Specific antibody responses to the peptide and each carrier protein were evaluated. While the antibody response to KLH was stronger than the response to BSA, both carrier proteins stimulated comparable antibody responses to the LH peptide. The immunostimulant proved to be more important for enhancing the LH peptide antibody response than the carrier protein selection; vaccines containing a combination of Aeromonas salmonicida and Vibrio anguillarum stimulated significantly greater LH peptide antibody production than any of the other three immunostimulants evaluated at 12 weeks post-vaccination. This study provides proof-of-concept for specific antibody production against a hapten-carrier protein antigen in Atlantic salmon and reinforces the importance of vaccine immunostimulant selection. |
| Databáze: | OpenAIRE |
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