Skin Epidermis and Adnexa Regrowth Induced by Treatment With Artificial Dermal Template After Full-Thickness Skin Wound
| Autor: | Pan Zhou, Kun Wang, Hugo Vankelecom, Gongchi Li, Jianghai Chen, Youtong Wu, Xiaobo Zhao, Hang Xue, Binghui Li, Yuxiong Weng |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Pathology artificial dermal template PROMOTER Rats Sprague-Dawley 030207 dermatology & venereal diseases Random Allocation 0302 clinical medicine Medicine Skin 0303 health sciences integumentary system SCAFFOLD Biopsy Needle Wnt signaling pathway skin defect General Medicine Skin Transplantation Immunohistochemistry medicine.anatomical_structure Stem cell medicine.symptom Wound edge Life Sciences & Biomedicine STEM-CELLS Hair Follicle EXPRESSION medicine.medical_specialty Dermatology Sensitivity and Specificity 03 medical and health sciences stem cells Full thickness skin Animals Regeneration biologics 030304 developmental biology Skin Artificial Wound Healing Science & Technology skin adnexa business.industry Regeneration (biology) HAIR FOLLICLE REGENERATION Rats Disease Models Animal regeneration Wounds and Injuries Surgery Epidermis Contracture business Wound healing |
| Zdroj: | The international journal of lower extremity wounds. 18(1) |
| ISSN: | 1552-6941 |
| Popis: | Full-thickness skin wounds are common accidents. Although healing can be achieved by treatments like autologous skin grafts, donor site morbidity is hardly evitable. In this article, we provide compelling evidence demonstrating that artificial dermal template (ADT)-treated wound healing is achieved by regrowth of skin epidermis as well as adnexa without skin grafts by use of rodent models. First, by fixating a chamber to the wound edge, we confirmed that wound healing was achieved by regeneration instead of contracture. We found highly proliferative cells in adnexa in the newly formed skin. In the distal edge of newly formed skin, we identified immature hair follicles at early developing stages, suggesting they were newly regenerated. Second, we observed that the Lgr5-positive hair follicle stem cells contributed to formation of new hair follicles through a lineage tracing model. Also, Lgr6-positive cells were enriched in distal edge of newly developed skin. Finally, WNT signaling pathway mediators were highly expressed in the new skin epidermis and adnexa, implying a potential role of WNT signaling during ADT treatment-stimulated skin regrowth. Taken together, our findings demonstrated that full skin regrowth can be induced by ADT treatment alone, thus arguing for its wide clinical application in skin wound treatment. ispartof: INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS vol:18 issue:1 pages:42-55 ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
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