Cytoprotective Encapsulation of Individual Jurkat T Cells within Durable TiO2 Shells for T-Cell Therapy
| Autor: | Gulaim A. Seisenbaeva, Mi-Hee Kim, Daewha Hong, Vadim G. Kessler, Insung S. Choi, Matthew Park, Eun Hyea Ko, Wongu Youn |
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| Rok vydání: | 2017 |
| Předmět: |
Adoptive cell transfer
Lymphokine-activated killer cell biology Chemistry CD3 T cell Lymphocyte medicine.medical_treatment Nanotechnology General Chemistry 02 engineering and technology General Medicine 010402 general chemistry 021001 nanoscience & nanotechnology Jurkat cells 01 natural sciences Catalysis Cell biology 0104 chemical sciences medicine.anatomical_structure Antigen Cancer immunotherapy biology.protein medicine 0210 nano-technology |
| Zdroj: | Angewandte Chemie. 129:10842-10846 |
| ISSN: | 0044-8249 |
| Popis: | Lymphocytes, such as T cells and natural killer (NK) cells, have therapeutic promises in adoptive cell transfer (ACT) therapy, where the cells are activated and expanded in vitro, and then infused into a patient. However, the in vitro preservation of labile lymphocytes during transfer, manipulation, and storage has been one of the bottlenecks in the development and commercialization of therapeutic lymphocytes. Herein, we suggest a "cell-in-shell" (a.k.a. artificial spore) strategy to enhance the cell viability in the practical settings, while maintaining biological activities for therapeutic efficacy. A durable titanium oxide (TiO2) shell is formed on individual Jurkat T cells, and the CD3 and other antigens on cell surfaces remain accessible to the antibodies. Interleukin-2 (IL-2) secretion is also not hampered by the shell formation. This work suggests a chemical toolbox for effectively preserving lymphocytes in vitro and developing the lymphocyte-based cancer immunotherapy. |
| Databáze: | OpenAIRE |
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