Brown bear sperm double freezing: Effect of elapsed time and use of PureSperm® gradient between freeze-thaw cycles
| Autor: | Luis Anel, L. Anel-López, S. Borragan, Paulino de Paz, Manuel Alvarez-Rodriguez, E. López-Urueña, Mercedes Alvarez, C. Martínez-Rodríguez |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Sociedad Regional Cántabra de Promoción Turística |
| Rok vydání: | 2013 |
| Předmět: |
Male
Semen Biology Semen analysis General Biochemistry Genetics and Molecular Biology Cryopreservation Semen collection Andrology Recovery rate Freezing medicine Animals Consecutive freezing–thawing cycles Sperm cryopreservation Acrosome medicine.diagnostic_test Ecology PureSperm General Medicine Gradient centrifugation Spermatozoa Sperm Semen Analysis Brown bear General Agricultural and Biological Sciences Ursidae |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1090-2392 0011-2240 |
| Popis: | The use of sexed spermatozoa has great potential to captive population management in endangered wildlife. The problem is that the sex-sorting facility is a long distance from the semen collection place and to overcome this difficulty two freeze-thaw cycles may be necessary. In this study, effects of refreezing on brown bear electroejaculated spermatozoa were analyzed. We carried out two experiments: (1) to assess the effects of the two freezing-thawing cycles on sperm quality and to analyze three different elapsed times between freezing-thawing cycles (30, 90 and 180. min), and (2) to analyze the use of PureSperm between freezing-thawing cycles to select a more motile and viable sperm subpopulation which better survived first freezing. The motility, viability and undamaged acrosomes were significantly reduced after the second thawing respect to first thawing into each elapsed time group, but the elapsed times did not significantly affect the viability and acrosome status although motility was damaged. Our results with the PureSperm gradient showed higher values of viability in freezability of select sample (pellet) respect to the rest of the groups and it also showed a significant decrease in the number of acrosome damaged. In summary, the double freezing of bear semen selected by gradient centrifugation is qualitatively efficient, and thus could be useful to carry out a sex-sorting of frozen-thawed bear spermatozoa before to send the cryopreserved sample to a biobank. Given the low recovery of spermatozoa after applying a selection gradient, further studies will be needed to increase the recovery rate without damaging of the cell quality. This work was supported in part by MICINN (CGL 2010-19213/BOS) and CANTUR S.A. |
| Databáze: | OpenAIRE |
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