Spatial Organization of Expanding Bacterial Colonies Is Affected by Contact-Dependent Growth Inhibition
| Autor: | A. Jamie Wood, Michael J. Bottery, Calvin Dytham, Ioannis Passaris, Marjan W. van der Woude |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Salmonella typhimurium competition systems Population level genetic structures media_common.quotation_subject Population Population Dynamics individual-based modeling Biology Models Biological General Biochemistry Genetics and Molecular Biology Competition (biology) Article 03 medical and health sciences chemistry.chemical_compound Human health 0302 clinical medicine Escherichia coli education Spatial organization media_common education.field_of_study Spatial Analysis Growth retardation Spatial structure Contact Inhibition Computational Biology spatial structure 030104 developmental biology chemistry Evolutionary biology Microbial Interactions Growth inhibition Microorganisms Genetically-Modified CDI General Agricultural and Biological Sciences competition 030217 neurology & neurosurgery contact-dependent inhibition type V secretion system |
| Zdroj: | Current Biology Bottery, M, Passaris, I, Dytham, C, Wood, A J & van der Woude, M 2019, ' Spatial Organization of Expanding Bacterial Colonies Is Affected by Contact-Dependent Growth Inhibition ', Current Biology, vol. 29, no. 21, pp. 3622-3634.E5 . https://doi.org/10.1016/j.cub.2019.08.074 |
| ISSN: | 1879-0445 0960-9822 |
| DOI: | 10.1016/j.cub.2019.08.074 |
| Popis: | Summary Identifying how microbes are able to manipulate, survive, and thrive in complex multispecies communities has expanded our understanding of how microbial ecosystems impact human health and the environment. The ability of bacteria to negatively affect neighbors, through explicit toxin delivery systems, provides them with an opportunity to manipulate the composition of growing microbial communities. Contact-dependent inhibition (CDI) systems (a Type Vb secretion system) are a distinct subset of competition systems whose contribution to shaping the development of spatially structured bacterial communities are yet to be fully understood. Here, we compare the impact of different CDI systems, at both the single-cell and population level, to determine the key drivers of CDI-mediated competition within spatially structured bacterial populations. Through an iterative approach using both an Escherichia coli experimental system and computational modeling, we show that CDI systems have subtle and system-specific effects at the single-cell level, generating single-cell-wide boundaries between CDI-expressing inhibitor cells and their neighboring targets. Despite the subtle effects of CDI at a single-cell level, CDI systems greatly diminished the ability of susceptible targets to expand their range during colony growth. The inoculum density of the population, together with the CDI system-specific variables of the speed of inhibition after contact and biological cost of CDI, strongly affects CDI-mediated competition. In contrast, the magnitude of the toxin-induced growth retardation of target cells only weakly impacts the composition of the population. Our work reveals how distinct CDI systems can differentially affect the composition and spatial arrangement of bacterial populations. Highlights • CDI causes subtle growth inhibition in a subset of contacted target cells • Model describes and predicts observed effects on spatial distribution of strains • CDI facilitates success of inhibitor strain increasing population patch size • A CDI system’s inhibition rate dominates toxicity in driving competition outcome Bottery et al. use an interdisciplinary approach to analyze contact-dependent inhibition in bacteria. CDI-dependent growth rate reduction is subtle but facilitates increased patch size for inhibitor cells in competitions on solid media. Of the variables impacting outcome, it is striking that the CDI system’s inhibition rate dominates toxicity. |
| Databáze: | OpenAIRE |
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