CMV70-3P miRNA contributes to the CMV mediated glioma stemness and represents a target for glioma experimental therapy
| Autor: | Anatoly Y. Baryshnikov, Kadagidze Zg, Ilya V. Ulasov, Dhimankrishna Ghosh, Charles Cobbs, Natalya V. Kaverina, Apollon I. Karseladze, M. A. Baryshnikova |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nephrology medicine.medical_specialty Pathology Brain tumor Cytomegalovirus Gene Expression 03 medical and health sciences 0302 clinical medicine Cell Movement Cancer stem cell Cell Line Tumor Internal medicine Neurosphere Glioma microRNA medicine Humans AC133 Antigen Promoter Regions Genetic miRNA business.industry SOXB1 Transcription Factors Cancer medicine.disease 3. Good health MicroRNAs Phenotype 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cytomegalovirus Infections glioma stem cells Neoplastic Stem Cells Cancer research RNA Viral Stem cell business brain tumor Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.11175 |
| Popis: | // Ilya V. Ulasov 1, 2, 3 , Natalya V. Kaverina 3, 4 , Dhimankrishna Ghosh 1 , Marya A. Baryshnikova 2, 3 , Zaira G. Kadagidze 3 , Apollon I. Karseladze 3 , Anatoly Y. Baryshnikov 2, 3 , Charles S. Cobbs 1 1 Swedish Neuroscience Institute, Center for Advanced Brain Tumor Treatment, Seattle, WA, 98122, USA 2 Institute of Experimental Diagnostics and Therapy of Tumors, N.N. Blokhin Russian Cancer Research Center, Moscow, 115478, Russia 3 NN. Blokhin Cancer Research Center, RAMN, Moscow, 115478, Russia 4 Current employment: Division of Nephrology, University of Washington, Seattle, 98109, WA, USA Correspondence to: Ilya V. Ulasov, email: Ilya.Ulasov@Swedish.org Charles S. Cobbs, email: Charles.Cobbs@Swedish.org Keywords: brain tumor, cytomegalovirus, glioma stem cells, miRNA Received: November 17, 2015 Accepted: July 26, 2016 Published: August 10, 2016 ABSTRACT Glioblastoma multiforme (GBM) is a rapidly progressive brain tumor with a median survival of 15–19 months. Therapeutic resistance and recurrence of the disease is attributed to cancer stem cells (CSC). Here, we report that CMV70-3P miRNA encoded by CMV increases GBM CSC stemness. Inhibition of CMV70-3P expression using oligo inhibitors significantly attenuated the ability of primary glioma cells to proliferate and form neurospheres. At the molecular level, we show that CM70-3P increases expression of cellular SOX 2. Collectively, these findings indicate that CMV70-3P is a potential regulator of CMV- mediated glioma progression and cancer stemness. |
| Databáze: | OpenAIRE |
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