Ionophore-based optical sensor for urine creatinine determination
| Autor: | Alfonso Salinas-Castillo, Miguel M. Erenas, Pascal Blondeau, Inmaculada Ortiz-Gomez, Pablo Ballester, Ignacio de Orbe-Payá, Daniel Hernández-Alonso, Luis Fermín Capitán-Vallvey, Francisco J. Andrade |
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| Rok vydání: | 2020 |
| Předmět: |
biofluids
Metabolite Ionophore disposable sensor optode Bioengineering 02 engineering and technology Urine Urinalysis Biofluid 01 natural sciences spectrometry chemistry.chemical_compound Limit of Detection pH indicator Wide dynamic range host−guest chemistry Humans Instrumentation Fluid Flow and Transfer Processes Creatinine Reproducibility Chromatography calix[6]pyrrole Ionophores Process Chemistry and Technology 010401 analytical chemistry Optical Devices Membranes Artificial Hydrogen-Ion Concentration Disposable sensor 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Calibration Optode 0210 nano-technology Host-guest chemistry |
| DOI: | 10.5281/zenodo.3766266 |
| Popis: | Creatinine is a metabolite present in urine, and its concentration is used to diagnose and monitor kidney performance. For that reason, the development of new sensors to analyze this metabolite and obtain accurate results in a short period of time is necessary. An optical disposable sensor for monitoring creatinine levels in urine is described. The system, based on a new aryl-substituted calix[4]pyrrole synthetic receptor, has an unusual coextraction scheme. Due to the low pKa values of creatininium (pKa 4.8), a careful selection of a lipophilic pH indicator that works in acid medium is required. The sensor components were optimized, and the new sensor displays a good response time to creatinine (approximately 3 min) over a wide dynamic range (from 1 × 10−5 to 1 × 10−2 M). Moreover, the optical selectivity coefficients obtained for creatinine over common cations present in urine meet the requirements for real sample measurements. With a good sensor-to-sensor reproducibility (RSD, 5.1−6.9% in the middle of the range), this method provides a simple, quick, cost-effective, and selective alternative to the conventional methodology based on Jaffé’s reaction FQM-118 MINECO (CTQ2016-78754-C2-1-R) Spanish Ministry of Economy and Competitiveness and European Regional Development Fund (ERDF) CTQ2016-77128-R MINECO (CTQ2017-84319-P) Severo Ochoa Excellence Accreditation 2014-2018 SEV-2013-0319 FEDER funds (Project CTQ2017- 84319-P) CERCA Programme/Generalitat de Catalunya and AGAUR (2017 SGR 1123) |
| Databáze: | OpenAIRE |
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