microRNA-589-5p modulates the expression of hemocyanin as part of the anti-WSSV immune response in Litopenaeus vannamei
| Autor: | Shengkang Li, Caihui Sun, Jude Juventus Aweya, Yueling Zhang, Yujian Hong, Shiyuan Bao, Zhihong Zheng, Defu Yao |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Hemocytes medicine.medical_treatment Immunology Litopenaeus Hepatopancreas Biology Arthropod Proteins Cell Line Microbiology 03 medical and health sciences White spot syndrome virus 1 0302 clinical medicine Immune system Penaeidae In vivo medicine Animals Gene knockdown Schneider 2 cells Hemocyanin biology.organism_classification DNA Virus Infections Immunity Innate Shrimp MicroRNAs 030104 developmental biology Gene Expression Regulation 030220 oncology & carcinogenesis Hemocyanins Drosophila Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 107:103642 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2020.103642 |
| Popis: | The respiratory glycoprotein, hemocyanin (HMC) has multiple immune-related functions, including antiviral activity. In this study, in silico methods were used to predict seven miRNAs targeting Litopenaeus vannamei HMC (LvHMC), out of which miR-589-5p was selected for further investigation because of its role in immune response. Transcript levels of miR-589-5p were ubiquitously distributed in all shrimp tissues examined, and significantly induced in hemocytes and hepatopancreas upon challenge with white-spot syndrome virus (WSSV) as well as by marine bacterial pathogens, which suggest that miR-589-5p is involved in shrimp immune response to pathogens. Morever, using Drosophila S2 cells stably overexpressing EGFP-LvHMC, flow cytometry and dual luciferase reporter assays, miR-589-5p was shown to significantly inhibit the in vitro expression of LvHMC. In addition, in vivo knockdown of miR-589-5p using antagomir-589-5p resulted in significant down-regulation in LvHMC expression, while overexpression of miR-589-5p using agomir-589-5p decreased the level of LvHMC expression in shrimp hemocytes and hepatopancreas. Further, the increased expression of miR-589-5p resulted in high shrimp mortality following WSSV challenge, coupled with an increase in the number of WSSV copies in hemocytes and hepatopancreas. These results suggest that miR-589-5p is involved in shrimp immune response to WSSV by negatively regulating the expression of LvHMC. |
| Databáze: | OpenAIRE |
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