Biofilms in orthopedic infections: a review of laboratory methods
| Autor: | Jeffrey F. Granger, Matthew C. Swearingen, Devendra H. Dusane, Paul Stoodley, Alex C. DiBartola |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty Prosthesis-Related Infections 030106 microbiology Bacterial Physiological Phenomena Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Revision Surgeries Humans Immunology and Allergy Medicine Intensive care medicine 030222 orthopedics Laboratory methods Bacteria Clinical Laboratory Techniques business.industry Bacterial Infections Prostheses and Implants General Medicine Surgery Orthopedics Biofilms Orthopedic surgery business |
| Zdroj: | APMIS. 125:418-428 |
| ISSN: | 0903-4641 |
| Popis: | Bacterial infection after hardware implantation in orthopaedic surgery is a devastating issue as it often necessitates increased hospital costs and stays, multiple revision surgeries, and prolonged use of antibiotics. Due to the nature of hardware implantation into the body, these infections are commonly in the form of attached biofilms. The current literature on a range of methodologies to study clinically explanted infected orthopaedic hardware, with potential biofilm, in the laboratory setting is limited. General methods include traditional and advanced culturing techniques, microscopy imaging techniques, and techniques that manipulate genetic material. The future of diagnostic techniques for infected implants, innovative hardware design, and treatment solutions for patients all depend on the successful evaluation and characterization of clinical samples in the laboratory setting. This review will provide an overview of current methods to study biofilms associated with orthopaedic infections, as well as provide insight into future directions in the field. |
| Databáze: | OpenAIRE |
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