Multiplexed serologic assay for nine anogenital human papillomavirus types
| Autor: | Richard Gesser, Richard M. Haupt, Tina Green, Mark T. Esser, Katie Matys, Alfred J. Saah, David Opalka, Paul Bojczuk, Frank J. Dutko |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Male Virosomes medicine.drug_class Clinical Biochemistry Immunology Monoclonal antibody Antibodies Viral Sensitivity and Specificity Immunoglobulin G Serology Antigen Virology medicine Immunology and Allergy Animals Humans Clinical Laboratory Immunology Papillomavirus Vaccines Antigens Viral Papillomaviridae Immunoassay biology medicine.diagnostic_test Papillomavirus Infections virus diseases Primary and secondary antibodies Macaca mulatta Titer biology.protein Female Antibody |
| Zdroj: | Clinical and vaccine immunology : CVI. 17(5) |
| ISSN: | 1556-679X |
| Popis: | A multiplexed human papillomavirus (HPV) immunoassay has been developed for the detection of human IgG antibodies to HPV type 6, 11, 16, 18, 31, 33, 45, 52, and 58 virus-like particle (VLP) types in serum following natural infection or immunization with VLP-based vaccines. The VLP antigens were covalently conjugated to carboxyl Luminex microspheres (MS) using a carbodiimide chemistry. Antibody (Ab) titers were determined in a direct binding format, in which an IgG1- to -4-specific, phycoerythrin (PE)-labeled monoclonal antibody (MAb) (HP6043) binds to human serum IgG antibodies. Pooled serum samples from rhesus macaques immunized with a 9-valent VLP-based vaccine served as the reference standard. The overall specificity of the assay was >99%, and the linearity (parallelism) of the assay was |
| Databáze: | OpenAIRE |
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