Potential bacterial biomarkers for insect colonization in forensic cases: preliminary quantitative data on Wohlfahrtiimonas chitiniclastica and Ignatzschineria indica dynamics
Autor: | Cristina Purcarea, Georgiana Necula-Petrareanu, Lavinia Iancu |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
DNA Bacterial Molecular biology 030106 microbiology lcsh:Medicine Zoology DNA gyrase Microbiology Article 03 medical and health sciences Bacterial Proteins Animals Colonization Calliphoridae lcsh:Science Pathogen Larva Multidisciplinary biology Host (biology) Diptera lcsh:R fungi Forensic Sciences food and beverages biology.organism_classification 030104 developmental biology DNA Gyrase Instar lcsh:Q Cattle Lucilia illustris Biomarkers Gammaproteobacteria |
Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) |
ISSN: | 2045-2322 |
Popis: | For the last decades, forensic microbiology became an emerging complementary tool in criminalistics. Although the insect-microbe interactions regarding pathogen transmission were extensively studied, only scarce information is available on bacterial transfer from necrophagous insects to host tissues. Our data provides the first report on the occurrence of Wohlfahrtiimonas chitiniclastica and Ignatzschineria indica in Lucilia illustris Meigen, 1826 (Diptera: Calliphoridae), and the quantitative dynamics of the two bacterial species along the insect life-stages and transfer to beef and pork host tissues using qPCR gyrase b specific primers. The content of both bacterial species increased along the insect life stages. W. chitiniclastica was detected in all developmental stages independent of the feeding substrate. I. indica was measurable with 102 gene copies ng−1 DNA threshold starting from the third instar larvae when feeding on beef, and from the egg stage with a 102× higher representation when using the pork substrate. The transfer of bacterial species to both tissues occurred after 3 colonization days except for I. indica that was visible in beef liver only during day 5. Considering the utilization of pork tissues as human analogues, these quantitative microbial dynamics data provides first insect-specific bacterial candidates as potential colonization biomarkers in forensic investigations. |
Databáze: | OpenAIRE |
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