Infarct Fibroblasts Do Not Derive From Bone Marrow Lineages
| Autor: | Ju Chen, Yusu Gu, Paola Cattaneo, Thomas Moore-Morris, Tatiana Kisseleva, Nuno Guimarães-Camboa, Julius Bogomolovas, Lunfeng Zhang, Sylvia M. Evans, Michel Pucéat, Mercedes Ricote, Marta Cedenilla, Indroneal Banerjee, Nancy D. Dalton, Kirk L. Peterson |
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| Přispěvatelé: | Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Skaggs School of Pharmacy and Pharmaceutical Sciences [San Diego], University of California [San Diego] (UC San Diego), University of California-University of California, Institute for Biomedical Sciences Abel Salazar and GABBA Graduate Program [Porto, Portugal], Universidade do Porto, Department of Medicine [San Diego], Department of Pharmacology [La Jolla, CA, USA], Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of California (UC)-University of California (UC), Universidade do Porto = University of Porto, Department of Medicine [Univ California San Diego] (MED - UC San Diego), School of Medicine [Univ California San Diego] (UC San Diego), University of California (UC)-University of California (UC)-University of California [San Diego] (UC San Diego), American Heart Association, Marie Curie, Unión Europea. Comisión Europea. 7 Programa Marco, Ministerio de Educación, Cultura y Deporte (España), Leducq Foundation, Fondation Leducq |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology Physiology Myocardial Infarction 030204 cardiovascular system & hematology Cardiorespiratory Medicine and Haematology Inbred C57BL Cardiovascular Mice 0302 clinical medicine Fibrosis Fibrocyte Medicine Myocardial infarction Myofibroblasts Cells Cultured Bone Marrow Transplantation Cultured heart diseases Artery ligation Haematopoiesis myocardial infarction medicine.anatomical_structure Heart Disease Cardiology and Cardiovascular Medicine Pericardium Bone marrow transplant medicine.medical_specialty bone marrow Cells Clinical Sciences Bone Marrow Cells Article Collagen Type I 03 medical and health sciences Lineage tracing fibroblasts Humans Animals Cell Lineage Heart Disease - Coronary Heart Disease business.industry Myocardium fibrosis medicine.disease Mice Inbred C57BL 030104 developmental biology [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics Cardiovascular System & Hematology Bone marrow business |
| Zdroj: | Circulation research, vol 122, iss 4 Circulation Research Circulation Research, American Heart Association, 2018, 122 (4), pp.583-590 Circulation Research, 2018, 122 (4), pp.583-590 Repisalud Instituto de Salud Carlos III (ISCIII) |
| ISSN: | 2008-0050 0009-7330 1524-4571 |
| Popis: | Myocardial infarction is a major cause of adult mortality worldwide. The origin(s) of cardiac fibroblasts that constitute the postinfarct scar remain controversial, in particular the potential contribution of bone marrow lineages to activated fibroblasts within the scar. The aim of this study was to establish the origin(s) of infarct fibroblasts using lineage tracing and bone marrow transplants and a robust marker for cardiac fibroblasts, the Collagen1a1-green fluorescent protein reporter. Using genetic lineage tracing or bone marrow transplant, we found no evidence for collagen-producing fibroblasts derived from hematopoietic or bone marrow lineages in hearts subjected to permanent left anterior descending coronary artery ligation. In fact, fibroblasts within the infarcted area were largely of epicardial origin. Intriguingly, collagen-producing fibrocytes from hematopoietic lineages were observed attached to the epicardial surface of infarcted and sham-operated hearts in which a suture was placed around the left anterior descending coronary artery. In this controversial field, our study demonstrated that the vast majority of infarct fibroblasts were of epicardial origin and not derived from bone marrow lineages, endothelial-to-mesenchymal transition, or blood. We also noted the presence of collagen-producing fibrocytes on the epicardial surface that resulted at least in part from the surgical procedure. T.M. Moore-Morris was supported by American Heart Association postdoctoral fellowship 11POST7310066. P. Cattaneo was supported by the Marie Curie International Outgoing Fellowship within the 7th European Community Framework Program under grant agreement No 623739—The Cardiac Code. M. Cedenilla received funding from the Spanish Ministry of Education, Culture, and Sports, Predoctoral Fellowship program (FPU, AP2008-00508) and Travel Grant Program (TTFPU11-AP2008-00508). J. Bogomolovas is supported by the European Commission’s Marie Sklodowska-Curie Individual Fellowship (Titin Signals, 656636). S.M. Evans is funded by grants from the National Heart, Lung, and Blood Institute and the Leducq Foundation. M. Pucéat and T.M. Moore-Morris acknowledge the generosity of the Leducq Foundation (SHAPEHEART). Sí |
| Databáze: | OpenAIRE |
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