Exploring the interaction between polystyrene nanoplastics and Allium cepa during germination: Internalization in root cells, induction of toxicity and oxidative stress
| Autor: | Stefania Bottega, Lorenza Bellani, Monica Ruffini Castiglione, Carmelina Spanò, Lucia Giorgetti, F. Barbieri, Simonetta Muccifora |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitotic index Physiology media_common.quotation_subject Germination Plant Science medicine.disease_cause Plant Roots 01 natural sciences Allium 03 medical and health sciences Allium cepa L. Genotoxicity Oxidative stress Polystyrene nanoplastics Ultrastructural analysis Ultrastructural analysis Onions Mitotic Index Genetics medicine Internalization Cytotoxicity Cellular compartment media_common Allium cepa L Chemistry Polystyrene nanoplastics technology industry and agriculture food and beverages 030104 developmental biology Oxidative stress Micronucleus test Biophysics Polystyrenes Genotoxicity Plastics 010606 plant biology & botany |
| Zdroj: | Plant physiology and biochemistry (Paris) 149 (2020): 170–177. doi:10.1016/j.plaphy.2020.02.014 info:cnr-pdr/source/autori:Lucia Giorgetti a1, Carmelina Spanò bc1, Simonetta Muccifora d, Stefania Bottega b, Francesco Barbieri d, Lorenza Bellani ad, Monica Ruffini Castiglione bc/titolo:Exploring the interaction between polystyrene nanoplastics and Allium cepa during germination: Internalization in root cells, induction of toxicity and oxidative stress/doi:10.1016%2Fj.plaphy.2020.02.014/rivista:Plant physiology and biochemistry (Paris)/anno:2020/pagina_da:170/pagina_a:177/intervallo_pagine:170–177/volume:149 |
| ISSN: | 0981-9428 |
| Popis: | With the aim to investigate the mechanisms of action of nano plastics (nano PS) on plants, seeds of Allium cepa were germinated for 72 h in the presence of polystyrene nano PS (50 nm size, at concentrations of 0.01, 0.1 and 1 g L−1) and, subsequently, roots were analysed by a multifaceted approach. No effect was induced by any concentration of nano PS on the percentage of seed germination while root growth was inhibited by 0.1 and 1 g L−1 nano PS. Cytological analysis of the root meristems indicated cytotoxicity (reduction of mitotic index) and genotoxicity (induction of cytogenetic anomalies and micronuclei) starting from the lowest dose. Moreover, the biochemical and histochemical analysis of oxidative stress markers gave evidence of stress induction, especially at the highest doses. Damages reported could be due to mechanical surface contact in root external layers, as evidenced by histological localization, and to the internalization of nano PS in different cellular compartments, observed under TEM. The present research underlines the hazardous nature of nano PS, that for their ability to be internalized into crop plants, can enter into different trophic levels of the food chain. |
| Databáze: | OpenAIRE |
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