Unleashing meiotic crossovers in hybrid plants
| Autor: | Mathilde Seguela-Arnaud, Joiselle Blanche Fernandes, Raphael Mercier, Cécile Larchevêque, Andrew H. Lloyd |
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| Přispěvatelé: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Université Paris-Sud - Paris 11 (UP11), Fondation Simone et Cino Del Duca/Institut de France, LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS], International Outgoing Fellowships [PIOF-GA-2013-628128 POLYMEIO], European Project: 281659,EC:FP7:ERC,ERC-2011-StG_20101109,MEIOSIGHT(2012) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Coefficient of coincidence 0106 biological sciences Crossover Arabidopsis Context (language use) Breeding Biology Genes Plant Genetic recombination 01 natural sciences 03 medical and health sciences Centimorgan crossovers Meiosis Centromere plant breeding meiosis [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Ectopic recombination FANCM Crossing Over Genetic Allele Homologous Recombination 030304 developmental biology Genetics 0303 health sciences Multidisciplinary fungi Wild type food and beverages Biological Sciences recombination 030104 developmental biology Mutation Recombination 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2018, 115 (10), pp.2431-2436. ⟨10.1073/pnas.1713078114⟩ Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (10), pp.2431-2436. ⟨10.1073/pnas.1713078114⟩ |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1101/159640 |
| Popis: | International audience; Meiotic crossovers shuffle parental genetic information, providing novel combinations of alleles on which natural or artificial selection can act. However, crossover events are relatively rare, typically one to three exchange points per chromosome pair. Recent work has identified three pathways limiting meiotic crossovers in Arabidopsis thaliana that rely on the activity of FANCM [Crismani W, et al. (2012) Science 336:1588-1590], RECQ4 [Seguela-Arnaud M, et al. (2015) Proc Natl Acad Sci USA 112:4713-4718], and FIGL1 [Girard C, et al. (2015) PLoS Genet 11:e1005369]. Here we analyzed recombination in plants in which one, two, or all three of these pathways were disrupted in both pure line and hybrid contexts. The greatest effect was observed when combining recq4 and figl1 mutations, which increased the hybrid genetic map length from 389 to 3,037 cM. This corresponds to an unprecedented 7.8-fold increase in crossover frequency. Disrupting the three pathways did not further increase recombination, suggesting that some upper limit had been reached. The increase in crossovers is not uniform along chromosomes and rises from centromere to telomere. Finally, although in wild type recombination is much higher in male meiosis than in female meiosis (490 cM vs. 290 cM), female recombination is higher than male recombination in recq4 figl1 (3,200 cM vs. 2,720 cM), suggesting that the factors that make wild-type female meiosis less recombinogenic than male wild-type meiosis do not apply in the mutant context. The massive increase in recombination observed in recq4 figl1 hybrids opens the possibility of manipulating recombination to enhance plant breeding efficiency. |
| Databáze: | OpenAIRE |
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