The effect of experimental conditions on the detection of spermine in cell extracts and tissues
| Autor: | Nandita M. deSouza, Nicholas G. Spencer, Thomas R. Eykyn, Geoffrey S. Payne |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cell Extracts
Male Magnetic Resonance Spectroscopy Spermidine Transplantation Heterologous Spermine chemistry.chemical_compound Mice Cell Line Tumor Putrescine Animals Humans Radiology Nuclear Medicine and imaging Perchloric acid Bovine serum albumin Spectroscopy Chromatography Aqueous solution biology Chemistry Tissue Extracts Transplantation Solutions Biochemistry biology.protein Metabolome Molecular Medicine Cattle Polyamine |
| Zdroj: | NMR in biomedicine. 23(2) |
| ISSN: | 1099-1492 |
| Popis: | The aim of this work was to investigate the effect of experimental conditions on the visibility of polyamines. In solution the chemical shift of the three groups of peaks (at approximately 1.8, 2.1 and 3.1 ppm) were found to be pH dependent. Relaxation times in aqueous solution at pH 7.0, 298 K and 11.74 T were measured to be: putrescine (T(1) = 2.49 s, T(2) = 2.07 s), spermidine (T(1) = 1.27 s, T(2) = 1.05 s) and spermine (T(1) = 1.02 s, T(2) = 0.82 s). Simple spin-echo sequences could not be used to measure T(2) as the spins also experience phase evolution from homonuclear coupling which imposes a modulation on the T(2) decay curve. This modulation is eliminated by using CPMG sequences with an echo spacing of |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text