Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples
| Autor: | S. I. Egglestone, W. G. Feng, B. S. W. Ho, B. K. C. Wong |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Molecular Sequence Data
medicine.disease_cause Polymerase Chain Reaction Pathology and Forensic Medicine Microbiology law.invention Species Specificity Urethra Antigen law medicine Humans Amino Acid Sequence Elisa method Polymerase chain reaction biology General Medicine biology.organism_classification Molecular biology Neisseria gonorrhoeae N gonorrhoeae Restriction enzyme Evaluation Studies as Topic Genes Bacterial Cervix Mucus Neisseriaceae Bacteria Research Article |
| Zdroj: | Journal of Clinical Pathology. 45:439-442 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jcp.45.5.439 |
| Popis: | AIMS: To evaluate the use of a cppB gene derived polymerase chain reaction (PCR) assay for direct detection of Neisseria gonorrhoeae in clinical samples. METHODS: A PCR assay was performed on 33 N gonorrhoeae strains and 12 other Neisseria species and other normal genital flora to evaluate the specificity of the chosen cppB primers. The assay was subsequently evaluated with 52 clinical swab samples collected from China. RESULTS: An amplified product of 390 base pairs (bp) was observed with all the N gonorrhoeae strains, each of these products on digestion with the restriction enzyme MspI produced two bands of 250 bp and 140 bp respectively. This set of primers did not produce any amplified product of the expected length with the other non-gonococcal strains tested. For the 52 clinical swabs, 34 were culture positive and PCR successfully detected all these positives. In addition the PCR was positive for two swabs which were culture negative but positive for N gonorrhoeae antigens when tested with the ELISA method (Gonozyme). CONCLUSIONS: This PCR assay is a promising diagnostic tool for detection of gonococci directly from clinical swab samples. Further evaluation is necessary. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|