How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
| Autor: | Yongqing Hua, Jin-Gao Yu, Yuping Tang, Pei Liu, KY Zhu, Jin-Ao Duan, Jianming Guo, Weiwei Tao, Yanyan Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Yuanhuacine Male medicine.medical_specialty MAP Kinase Signaling System medicine.medical_treatment Science Down-Regulation Traditional Chinese medicine Pharmacology Kidney Glycyrrhizae Radix Article 03 medical and health sciences Mice Network pharmacology Internal medicine Chinese traditional medicine Animals Medicine Chinese Traditional Diuretics Mice Inbred ICR Multidisciplinary Aquaporin 2 business.industry Kidney metabolism Up-Regulation 030104 developmental biology Endocrinology Toxicity Medicine Plant Preparations Diuretic business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| Popis: | As recorded in Traditional Chinese Medicine (TCM) theory, Gancao (Glycyrrhizae Radix et Rhizoma) could weaken the pharmacological effect or increase the toxicity of Yuanhua (Genkwa Flos). However, the theory has been suspected due to lack of evidence. Here, we investigate whether Gancao could weaken Yuanhua’s diuretic effect, if so, which chemicals and which targets may be involved. Results showed that Yuanhua exerted diuretic effect through down-regulating renal AQP 2, without electrolyte disturbances such as K+ loss which has been observed as side-effect of most diuretics. Gancao had no diuretic effect, but could impair Yuanhua’s diuretic effect through up-regulating renal AQP 2. Glycyrrhetinic acid (GRA) in Gancao could up-regulate AQP 2 and counteract the AQP 2 regulation effect of Yuanhuacine (YHC) and Ginkwanin (GKW) in Yuanhua. Network pharmacology method suggested that YHC, GKW and GRA could bind to MEK1/FGFR1 protein and influence ERK-MAPK pathway, which was verified by Western blotting. This study supports TCM theory and reminds that more attention should be paid to the safety and efficacy problems induced by improper combination between herbs. Moreover, we suggested that promising diuretics with less side effects can be developed from Chinese Medicines such as Yuanhua. |
| Databáze: | OpenAIRE |
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