LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer

Autor:	Hua Luo, Bing Jiang, Yu Kun Zhang, Guang Mei Qin, Li li Miao
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine Cancer Research Lung Neoplasms Survival Cell Biology Transfection 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Western blot Acetyltransferases Cell Movement ROCK1 Genetics medicine Humans Neoplasm Invasiveness RNA Antisense Luciferase Lung cancer RC254-282 Messenger RNA medicine.diagnostic_test Small cell lung cancer Intracellular Signaling Peptides and Proteins Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Small Cell Lung Carcinoma respiratory tract diseases MicroRNAs 030104 developmental biology medicine.anatomical_structure miR-148a Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research RNA Long Noncoding MCM3AP-AS1 Research Article
Zdroj:	BMC Cancer, Vol 21, Iss 1, Pp 1-8 (2021) BMC Cancer
ISSN:	1471-2407
Popis:	Background MCM3AP-AS1 is a recently characterized lncRNA playing an oncogenic role in several cancers. However, its role in lung cancer remains unknown. Here, we aimed to explore the functions of MCM3AP-AS1 in small cell lung cancer (SCLC) and the possible underlying mechanisms. Methods MCM3AP-AS1 and ROCK1 levels in SCLC patients were analyzed by qPCR. RNA pull-down and luciferase assays were performed to analyze the interaction between MCM3AP-AS1 and miR-148a. ROCK1 mRNA and protein levels were detected by qPCR and Western blot, respectively. Cell invasion and migration were analyzed by Transwell assays. Results MCM3AP-AS1 was upregulated in patients with SCLC, and a high MCM3AP-AS1 level was accompanied by a low survival rate. The binding of MCM3AP-AS1 to miR-148a predicted by bioinformatics analysis was verified by RNA pull-down and luciferase assays. However, MCM3AP-AS1 and miR-148a did not affect each other’s expression. ROCK1 was upregulated in SCLC tissues and positively correlated with MCM3AP-AS1. In SCLC cells, MCM3AP-AS1 overexpression increased ROCK1 and promoted cancer cell invasion and migration, while miR-148a overexpression showed the opposite effects and attenuated the effects of MCM3AP-AS1 overexpression on ROCK1 expression and cell behaviors. Conclusions MCM3AP-AS1 sponges miR-148a, thereby increasing SCLC cell invasion and migration via upregulating ROCK1 expression.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::926764bf65286b5587506e4605271c80 https://doaj.org/article/01b54ec498d74bdab2d8329daf638f27 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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