3D Hydrogels Containing Interconnected Microchannels of Subcellular Size for Capturing Human Pathogenic Acanthamoeba Castellanii
| Autor: | Katharina Siemsen, Christine Selhuber-Unkel, Yogendra Kumar Mishra, Anneke Möhring, Rainer Adelung, Sören B. Gutekunst, Steven Huth, Leonard Siebert, Michael Timmermann, Ingo Paulowicz, Britta Hesseler |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
cell migration Technische Fakultät 0206 medical engineering Polyacrylamide Biomedical Engineering Nanotechnology 02 engineering and technology Interconnectivity Article porous hydrogel Biomaterials chemistry.chemical_compound ddc:6 Microchannel Faculty of Engineering article human pathogens 021001 nanoscience & nanotechnology 020601 biomedical engineering 3D ceramic templates microchannels Template chemistry Self-healing hydrogels Acanthamoeba castellanii ddc:620 0210 nano-technology Porous hydrogel |
| Zdroj: | ACS Biomaterials Science & Engineering ACS Biomaterials Science & Engineering.-Washington : ACS Publications; 2019.-S.1784-1792. |
| Popis: | Porous hydrogel scaffolds are ideal candidates for mimicking cellular microenvironments, regarding both structural and mechanical aspects. We present a novel strategy to use uniquely designed ceramic networks as templates for generating hydrogels with a network of interconnected pores in the form of microchannels. The advantages of this new approach are the high and guaranteed interconnectivity of the microchannels, as well as the possibility to produce channels with diameters smaller than 7 μm. Neither of these assets can be ensured with other established techniques. Experiments using the polyacrylamide substrates produced with our approach have shown that the migration of human pathogenic Acanthamoeba castellanii trophozoites is manipulated by the microchannel structure in the hydrogels. The parasites can even be captured inside the microchannel network and removed from their incubation medium by the porous polyacrylamide, indicating the huge potential of our new technique for medical, pharmaceutical, and tissue engineering applications. |
| Databáze: | OpenAIRE |
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