Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor
| Autor: | Eriko Takano, Sastia Prama Putri, Rainer Breitling, Eiichiro Fukusaki, Francesco Del Carratore, Katsuaki Nitta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Histology lcsh:Biotechnology Biomedical Engineering Bioengineering Streptomyces coelicolor 02 engineering and technology Secondary metabolite Actinorhodin 03 medical and health sciences chemistry.chemical_compound transcriptomics cAMP lcsh:TP248.13-248.65 Extracellular medicine Cyclic adenosine monophosphate Purine metabolism biology Chemistry Cell growth secondary metabolites actinorhodin 021001 nanoscience & nanotechnology biology.organism_classification metabolomics 3. Good health 030104 developmental biology Biochemistry 0210 nano-technology Intracellular Biotechnology medicine.drug |
| Zdroj: | Nitta, K, Carratore, F D, Breitling, R, Takano, E, Putri, S P & Fukusaki, E 2020, ' Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor ', Frontiers in Bioengineering and Biotechnology, vol. 8 . https://doi.org/10.3389/fbioe.2020.595552 Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) Frontiers in Bioengineering and Biotechnology |
| Popis: | Cyclic adenosine monophosphate (cAMP) has been known to play an important role in regulating morphological development and antibiotic production in Streptomyces coelicolor. However, the functional connection between cAMP levels and antibiotic production and the mechanism by which cAMP regulates antibiotic production remain unclear. In this study, metabolomics- and transcriptomics-based multi-omics analysis was applied to S. coelicolor strains that either produce the secondary metabolite actinorhodin (Act) or lack most secondary metabolite biosynthesis pathways including Act. Comparative multi-omics analysis of the two strains revealed that intracellular and extracellular cAMP abundance was strongly correlated with actinorhodin production. Notably, supplementation of cAMP improved cell growth and antibiotic production. Further multi-omics analysis of cAMP-supplemented S. coelicolor cultures showed an increase of guanine and the expression level of purine metabolism genes. Based on this phenomenon, supplementation with 7-methylguanine, a competitive inhibitor of reactions utilizing guanine, with or without additional cAMP supplementation, was performed. This experiment revealed that the reactions inhibited by 7-methylguanine are mediating the positive effect on growth and antibiotic production, which may occur downstream of cAMP supplementation. |
| Databáze: | OpenAIRE |
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