Role of MMP-9 in epithelial-mesenchymal transition of thyroid cancer
| Autor: | Feng Wang, Yuanchun Li, Tiejun Li, Jing He, Xin Wang, Fan Yang, Chunyang Zhao, Chunling Feng |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Stromal cell lcsh:Surgery Vimentin lcsh:RC254-282 Thyroid cancer Transforming Growth Factor beta1 Small hairpin RNA 03 medical and health sciences 0302 clinical medicine Invasion Cell Movement Cell Line Tumor Humans Cytotoxic T cell Medicine Thyroid Neoplasms Epithelial–mesenchymal transition Migration biology business.industry Research Cell migration lcsh:RD1-811 Transfection Cadherins Prognosis Epithelial-mesenchymal transition lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology Matrix Metalloproteinase 9 Oncology Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research Surgery MMP-9 business |
| Zdroj: | World Journal of Surgical Oncology, Vol 18, Iss 1, Pp 1-9 (2020) World Journal of Surgical Oncology |
| ISSN: | 1477-7819 |
| DOI: | 10.1186/s12957-020-01958-w |
| Popis: | BackgroundThe purpose of this study is to explore the role and mechanism of MMP-9 in the EMT process of thyroid cancer (TC), so as to provide a basis for clinical exploration of invasion and metastasis process of TC, looking for biological markers of tumor metastasis and molecular intervention therapy.MethodsWestern blot and RT-PCR were employed to detect the expression of MMP-9 in human normal thyroid cell line HT-ori3 and human TC cell lines IHH-4 (PTC), FTC-133, and 8505C. Expression levels of EMT-related markers: epithelial cell marker E-cadherin and stromal cell marker Vimentin in TGF-1-induced TC cell lines were detected by Western blot and RT-PCR, respectively. The effects of MMP-9 downregulation on cell invasion and metastasis were investigated by wound-healing assay and cell invasion experiment.ResultsThe protein and mRNA expression levels of MMP-9 in TC cell lines were increased compared with the human normal thyroid cell line HT-ori3. When TGF-β1 was added, the expression of EMT and Vimentin increased while the expression of E-cadherin decreased. Compared with the control group, the TC cells stably transfected with MMP-9 shRNA showed inhibited EMT, decreased Vimentin expression, and increased E-cadherin expression. The induction of TGF-β1 did not promote the occurrence of EMT in TC cells which were stably transformed with MMP-9 shRNA. The addition of TGF-β1 to TC cells increased the ability of the cells to migrate and invade. Compared with the control group, the migration and invasion ability of TC cells stably transfected with MMP-9 shRNA was significantly reduced, and the induction of TGF-β1 could not restore the migration and invasion ability of cells without MMP-9.ConclusionsIn conclusion, we found that MMP-9 can be used as a biomarker for TC, which can promote the EMT process of TGF-β1 induced TC, and thus affecting the cell migration and invasion ability. |
| Databáze: | OpenAIRE |
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