Evidence for karyoplasmic homeostasis during endoreduplication and a ploidy-dependent increase in gene transcription during tomato fruit growth

Autor: Spencer Brown, Matthieu Bourdon, Catherine Cheniclet, Christian Chevalier, Mickael Bourge, Olivier Coriton, Jean Pierre Renaudin, Nathalie Frangne, Adeline Moïse, Valérie Rouyère, Julien Pirrello, Martine Peypelut
Přispěvatelé: Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Toulouse-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Amélioration des Plantes et Biotechnologies Végétales (APBV), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), UMR 619 Equipe Biologie de la Vigne, Université Sciences et Technologies - Bordeaux 1, Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), French Agence Nationale de la Recherche [ANR-09-GENM-105], Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse (ENSAT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université Sciences et Technologies - Bordeaux 1 (UB), ANR-09-JCJC-0110,BACH(2009)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
0106 biological sciences
Transcription
Genetic
Mitochondrion
01 natural sciences
MESH: Gene Amplification
Solanum lycopersicum
Transcription (biology)
MESH: Lycopersicon esculentum
Homeostasis
MESH: In Situ Hybridization
Fluorescence
MESH: Polytene Chromosomes
In Situ Hybridization
Fluorescence
MESH: Cell Size
Polytene Chromosomes
Genetics
0303 health sciences
Polytene chromosome
Fruit development
Endoreduplication
Chromatin
Mitochondria
Cell biology
MESH: Homeostasis
MESH: Microscopy
Electron
Transmission
Ploidy
MESH: Fruit
Transcription
Transcriptional Activation
MESH: Cell Nucleus
MESH: Mitochondria
MESH: Nucleolus Organizer Region
Mitosis
Biology
Tomato
MESH: Chromatin
Polyploidy
03 medical and health sciences
Microscopy
Electron
Transmission
MESH: Polyploidy
Organelle
Nucleolus Organizer Region
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
RNA
Messenger
Nuclear structure
Molecular Biology
Cell Size
030304 developmental biology
MESH: RNA
Messenger
Cell Nucleus
MESH: Transcription
Genetic
Gene Amplification
MESH: Mitosis
RNA
Ribosomal
Fruit
MESH: RNA
Ribosomal
MESH: Transcriptional Activation
010606 plant biology & botany
Developmental Biology
MESH: Endoreduplication
Zdroj: Development (Cambridge, England)
Development (Cambridge, England), Company of Biologists, 2012, 139 (20), pp.3817-26. ⟨10.1242/dev.084053⟩
Development (Cambridge, England), 2012, 139 (20), pp.3817-26. ⟨10.1242/dev.084053⟩
ISSN: 0950-1991
1477-9129
DOI: 10.1242/dev.084053⟩
Popis: International audience; Endopolyploidy is a widespread process that corresponds to the amplification of the genome in the absence of mitosis. In tomato, very high ploidy levels (up to 256C) are reached during fruit development, concomitant with very large cell sizes. Using cellular approaches (fluorescence and electron microscopy) we provide a structural analysis of endoreduplicated nuclei at the level of chromatin and nucleolar organisation, nuclear shape and relationship with other cellular organelles such as mitochondria. We demonstrate that endopolyploidy in pericarp leads to the formation of polytene chromosomes and markedly affects nuclear structure. Nuclei manifest a complex shape, with numerous deep grooves that are filled with mitochondria, affording a fairly constant ratio between nuclear surface and nuclear volume. We provide the first direct evidence that endopolyploidy plays a role in increased transcription of rRNA and mRNA on a per-nucleus basis. Overall, our results provide quantitative evidence in favour of the karyoplasmic theory and show that endoreduplication is associated with complex cellular organisation during tomato fruit development.
Databáze: OpenAIRE
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