Metabolic regulation rather than de novo enzyme synthesis dominates the osmo-adaptation of yeast

Autor: Karen van Eunen, Barbara M. Bakker, Jildau Bouwman, Marco Siderius, Alexander Lindenbergh, J. Kiewiet
Přispěvatelé: Molecular Cell Physiology, Medicinal chemistry, AIMMS, Center for Liver, Digestive and Metabolic Diseases (CLDM), Lifestyle Medicine (LM)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Glycerol
EXPRESSION
STEADY-STATE
GLYCEROL RESPONSE PATHWAY
Osmotic shock
Saccharomyces cerevisiae
Biomedical Innovation
Bioengineering
Applied Microbiology and Biotechnology
Biochemistry
SACCHAROMYCES-CEREVISIAE
BAKERS-YEAST
chemistry.chemical_compound
Life
Osmotic Pressure
Gene Expression Regulation
Fungal
Genetics
RNA
Messenger
OXIDATIVE STRESS
Biology
Osmotic concentration
biology
ACTIVATED PROTEIN-KINASE
Metabolism
biology.organism_classification
Adaptation
Physiological
Yeast
Glycerol-3-phosphate dehydrogenase
MSB - Microbiology and Systems Biology
chemistry
regulation analysis
gene expression
GROWTH
metabolic regulation
osmotic stress
EELS - Earth
Environmental and Life Sciences
Flux (metabolism)
Healthy Living
GLYCEROL-3-PHOSPHATE DEHYDROGENASE
CHEMOSTAT CULTURES
Signal Transduction
Biotechnology
Zdroj: Bouwman, J, Kiewiet, J A L, Lindenbergh, A, Van Eunen, K, Siderius, M H & Bakker, B M 2011, ' Metabolic regulation rather than de novo enzyme synthesis dominates the osmo-adaptation of yeast. ', Yeast, vol. 28, pp. 43-53 . https://doi.org/10.1002/yea.1819
Yeast, 28, 43-53. John Wiley and Sons Ltd
Yeast, 28(1), 43-53
Yeast, 1, 28, 43-53
ISSN: 0749-503X
DOI: 10.1002/yea.1819
Popis: Intracellular accumulation of glycerol is essential for yeast cells to survive hyperosmotic stress. Upon hyperosmotic stress the gene expression of enzymes in the glycerol pathway is strongly induced. Recently, however, it was shown that this gene-expression response is not essential for survival of an osmotic shock [Mettetal JT et al. (2008) Science 319: 482-484 and Westfall PJ et al. (2008) Proc Natl Acad Sci 105: 12212-12217]. Instead, pure metabolic adaptation can rescue the yeast. The existence of two alternative mechanisms urged the question which of these mechanisms dominates time-dependent adaptation of wild-type yeast to osmotic stress under physiological conditions. The regulation of the glycerol pathway was analysed in aerobic, glucose-limited cultures upon addition of 1 M of sorbitol, leading to a hyperosmotic shock. In agreement with earlier studies, the mRNA levels of the glycerol-producing enzymes as well as their catalytic capacities increased. Qualitatively this induction followed a similar time course to the increase of the glycerol flux. However, a quantitative regulation analysis of the data revealed an initial regulation by metabolism alone. After only a few minutes gene expression came into play, but even after an hour, 80% of the increase in the glycerol flux was explained by metabolic changes in the cell, and 20% by induction of gene expression. This demonstrates that the novel metabolic mechanism is not just a secondary rescue mechanism, but the most important mechanism to regulate the glycerol flux under physiological conditions. Copyright (C) 2010 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
Externí odkaz: