Evaluation of the diagnostic utility of a whole-blood interferon-gamma assay for determining the risk of exposure to Mycobacterium tuberculosis in Bacille Calmette-Guerin (BCG)-vaccinated individuals
| Autor: | Jin Hong Min, Sang Nae Cho, Soo Hee Hwang, Seok Yong Eum, Laura E. Via, Clifton E. Barry, Ye Jin Lee, Hyun Kyung Kwak |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Adult Male Tuberculosis Students Medical medicine.medical_treatment Nurses Article Mycobacterium tuberculosis Interferon-gamma Antigen Interferon medicine Humans Interferon gamma Whole blood Mycobacterium bovis biology business.industry Tuberculin Test General Medicine Middle Aged biology.organism_classification medicine.disease bacterial infections and mycoses Infectious Diseases Cytokine Immunology Female business medicine.drug |
| Zdroj: | Diagnostic microbiology and infectious disease. 61(2) |
| ISSN: | 0732-8893 |
| Popis: | We evaluated the utility of the "QuantiFERON-TB Gold In-Tube" (QuantiFERON) test that uses tuberculosis (TB)-specific antigens for the diagnosis of latent infection in such individuals. We also examined the correlation between the interferon (IFN)-gamma response to these antigens and the exposure risk to TB by evaluating antigen-specific IFN-gamma release in comparison with IFN-gamma release in response to purified protein derivative (PPD) in 3 groups: medical students, nurses in a TB hospital, and TB patients. All nurses and TB patients responded to PPD, whereas 52% (P0.0001) and 79.2% (P = 0.04) responded to QuantiFERON, respectively. In the medical students, only 10.4% responded to QuantiFERON, whereas 85.2% were positive to PPD (P0.0001). There was also a significant correlation between the levels of IFN-gamma production and the duration of employment in the group of nurses at the TB hospital, suggesting ongoing exposure in this high-risk group. Thus, these results demonstrate that Mycobacterium tuberculosis-specific IFN-gamma release assay accurately discriminates low- and high-risk healthy subjects and might therefore be a useful diagnostic tool for the diagnosis of latent infection in Bacille Calmette-Guerin (BCG)-vaccinated individuals. |
| Databáze: | OpenAIRE |
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