High throughput small RNA and transcriptome sequencing reveal capacitation-related microRNAs and mRNA in boar sperm
| Autor: | Wen-Cheng He, Ming-Xia Ran, Kai Liang, Rong-Hong Li, Ming Zhang, Yan Zhang, Ying-Nan Ren, Guang-Bin Zhou, Izhar Hyder Qazi, Yuan Li, Changjun Zeng |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Small RNA endocrine system lcsh:QH426-470 Swine lcsh:Biotechnology Biology Proteomics Transcriptome 03 medical and health sciences Capacitation lcsh:TP248.13-248.65 Boar Genetics medicine Animals High throughput sequencing RNA Messenger reproductive and urinary physiology Messenger RNA urogenital system Gene Expression Profiling RNA High-Throughput Nucleotide Sequencing Sperm capacitation Oocyte Sperm Spermatozoa Cell biology lcsh:Genetics MicroRNAs 030104 developmental biology medicine.anatomical_structure Gene Ontology miRNAs Biotechnology Research Article |
| Zdroj: | BMC Genomics BMC Genomics, Vol 19, Iss 1, Pp 1-12 (2018) |
| ISSN: | 1471-2164 |
| Popis: | Background Capacitation, a prerequisite for oocyte fertilization, is a complex process involving series of structural and functional changes in sperms such as membrane modifications, modulation of enzyme activities, and protein phosphorylation. In order to penetrate and fertilize an oocyte, mammalian sperms must undergo capacitation. Nevertheless, the process of sperm capacitation remains poorly understood and requires further elucidation. In the current study, via high throughput sequencing, we identified and explored the differentially expressed microRNAs (miRNAs) and mRNAs involved in boar sperm capacitation. Results We identified a total of 5342 mRNAs and 204 miRNAs that were differentially expressed in fresh and capacitated boar sperms. From these, 12 miRNAs (8 known and 4 newly identified miRNAs) and their differentially expressed target mRNAs were found to be involved in sperm capacitation-related PI3K-Akt, MAPK, cAMP-PKA and Ca2+signaling pathways. Conclusions Our study is first to provide the complete miRNA and transcriptome profiles of boar sperm. Our findings provide important insights for the understanding of the RNA profile in boar sperm and future elucidation of the underlying molecular mechanism relevant to mammalian sperm capacitation. Electronic supplementary material The online version of this article (10.1186/s12864-018-5132-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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